Integrative analysis of the metabolome and transcriptome provides insights into the mechanisms of flavonoid biosynthesis in blackberry. (March 2022)

• Record Type: Journal Article
• Authors: Wu, Yaqiong; Zhang, Chunhong; Huang, Zhengjin; Lyu, Lianfei; Li, Weilin; Wu, Wenlong
• Is Part Of: Food research international. Volume 153(2022)
• Publication Date: 2022-03
• Subjects: Differentially expressed metabolites -- Differentially expressed genes -- Coexpression network -- KEGG metabolism -- Fruit -- Transcription factor; DEMs differentially expressed metabolites -- DEGs differentially expressed genes -- FAO United Nations Food and Agriculture Organization -- CHS chalcone synthase -- CHI chalcone isomerase -- F3H flavanone 3-hydroxylase -- F3′H flavonoid 3′-hydroxylase -- ANS anthocyanidin synthase -- TFs transcription factors -- BU unripe fruits -- BR ripe fruits -- PCA principal component analysis -- PLS-DA partial least squares discriminant analysis -- OPLS-DA orthogonal projection to latent structures-discriminant analysis -- KEGG Kyoto Encyclopedia of Genes and Genomes -- GO Gene Ontology -- Nr nonredundant protein -- CYP73A trans-cinnamate 4-monooxygenase -- HKT shikimate O-hydroxycinnamoyltransferase -- PGT1 phlorizin synthase -- DFR bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase -- FLS flavonol synthase -- LAR leucoanthocyanidin reductase -- ANR anthocyanidin reductase -- BZ1 anthocyanidin 3-O-glucosyltransferase -- FG3 flavonol-3-O-glucoside/galactoside glucosyltransferase -- FPKM fragments per kilobase per million reads -- MBW MYB-bHLH-WD40 -- tt Arabidopsis transparent testa -- bZIP basic region/leucine zipper; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2022.110948
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: A comprehensive analysis of blackberry flavonoid biosynthesis and metabolism. Eight DEMs and 16 DEGs were annotated to flavonoid biosynthesis pathway. The study provides a reference for blackberry-related pharmaceutical industry. Abstract: Blackberry fruit is rich in anthocyanins, showing incomparable nutritional and health value. Anthocyanins are flavonoids that are downstream products of the flavonoid biosynthesis pathway. They are important secondary metabolites with antioxidant and anticancer functions. Therefore, we performed combined transcriptome and metabolome analyses of unripe and ripe blackberry fruits to identify the molecular mechanisms of flavonoid biosynthesis. Herein, we identified 997 differentially expressed metabolites (DEMs) in positive-ion mode, 411 DEMs in negative-ion mode, and 17, 566 differentially expressed genes (DEGs). Eight DEMs and 16 DEGs were annotated to the flavonoid biosynthesis pathway. Four DEGs related to flavonoids (unigene 0028088, unigene 0039337, unigene 0063209, and unigene 0014842) were positively correlated with related flavonoid DEMs. Interestingly, 8 DEGs and 11 flavonoid-related DEMs showed significant negative correlations. This study lays a foundation for further research on the biosynthesis and metabolism of flavonoid, providing a reference for the future breeding and cultivation of excellent high-flavonoid germplasm resources and the blackberry-related pharmaceutical industry.