Changes in Metabolic Regulation and the Microbiota Composition after Supplementation with Different Fatty Acids in db/db Mice. (7th January 2022)

• Record Type: Journal Article
• Authors: Martínez-Carrillo, Beatriz Elina; Mondragón-Velásquez, Talia; Ramírez-Durán, Ninfa; Aguirre-Garrido, José Félix; Valdés-Ramos, Roxana; Guadarrama-López, Ana Laura; Castillo-Cardiel, Arturo
• Other Names: Yan Zheng-Fei Academic Editor.
• Is Part Of: International journal of food science. Volume 2022(2022)
• Publication Date: 2022-01-07
• Subjects: Food -- Periodicals; Food industry and trade -- Periodicals; Food; Food Industry; Food; Food industry and trade; Periodicals; Electronic journals; Periodicals; Electronic journals; 664
• DOI: 10.1155/2022/3336941
• Languages: English
• ISSNs: 2356-7015

Abstract

Abstract : Introduction . The effects of fatty acids on health vary and depend on the type, amount, and route of consumption. EPA and DHA have a defined role in health, unlike coconut oil. Objective . The aim was to investigate the changes in metabolic regulation and the composition of the culture-dependent microbiota after supplementation with different fatty acids in db/db mice. Material and Methods . We were using 32 8-week-old db/db mice, supplemented for eight weeks with EPA/DHA derived from microalgae as well as coconut oil. The lipid, hormonal profiles, and composition of the culture-dependent microbiota and the phylogenetic analysis based on the 16S rRNA gene sequencing were determined for identification of the intestinal microbiota. Results . Enriched diet with EPA/DHA reduced TNF- α, C-peptide, insulin resistance, resistin, and the plasma atherogenic index, but increased TC, LDL-c, VLDL-c, and TG without changes in HDL-c. Coconut oil raised the HDL-c, GIP, and TNF- α, with TG, insulin resistance, adiponectin, and C-peptide reduced. Conclusion . The most abundant microbial populations were Firmicutes and the least Proteobacteria . EPA/DHA derived from microalgae contributes to improving the systemic inflammatory status, but depressed the diversity of the small intestine microbiota. Coconut oil only decreased the C-peptide, raising TNF- α, with an unfavorable hormonal and lipid profile.