Extraction, isolation and identification of four phenolic compounds from Pleioblastus amarus shoots and their antioxidant and anti-inflammatory properties in vitro. (16th April 2022)

• Record Type: Journal Article
• Authors: Ao, Xiaolin; Yan, Junling; Liu, Shuliang; Chen, Shujuan; Zou, Likou; Yang, Yong; He, Li; Li, Shanshan; Liu, Aiping; Zhao, Ke
• Is Part Of: Food chemistry. Volume 374(2022)
• Publication Date: 2022-04-16
• Subjects: Pleioblastus amarus shoots -- Polyphenols -- Structural elucidation -- Antioxidant capacity -- Anti-inflammatory; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2021.131743
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: Four phenolic compounds of the shoots of Pleioblastus amarus were isolated and identified for the first time. All the four compounds showed significant antioxidant capacity. Compound 2 (3-O-feruloyquinic acid) had strongest antioxidant capacity, but was unstable. Compound 2 inhibit IL-6, 1L-1β, iNOS, COX-2 and NF-κB production by RAW264.7 cells. Abstract: Pleioblastus amarus ( P. amarus ) shoots, belong to the grass family Gramineae, a traditional green vegetable in China, are rich in nutritional properties, and can provide various health benefits. This study isolated four compounds, namely (1–4), 3-O-coumaroylquinic acid (1), 3-O-feruloylquinic acid (2), 4-O-feruloylquinic acid (3), and 5-O-feruloylquinic acid (4) from Pleioblastus amarus shoots for the first time. The structures of the extracted compounds were determined using detailed spectroscopic (1D/2D NMR), high resolution electrospray ionization mass spectrometry (HR-ESI-MS), and infrared (IR) spectroscopy. The antioxidant capacity of 3-O-feruloylquinic acid (2) was stronger than that of the other compounds, while it also exhibited anti-inflammatory activity, significantly restricting the release of nitric oxide (NO) by lipopolysaccharide (LPS)-induced RAW 264.7 cells, displaying an inhibitory rate of 60.92 percent at a concentration of 400 μg/mL. Furthermore, 3-O-feruloylquinic acid (2) inhibited interleukin-1β ( IL-1β ), interleukin-6 ( IL-6 ), inducible nitric oxide synthase ( iNOS ), cyclooxygenase-2 ( COX-2 ), and nuclear factor-κB ( NF-κB ) expression and may be useful for developing novel antioxidant and anti-inflammatory substances.