Identification by volatolomics of hydrocarbon, oxygenated, sulfur and aromatic markers of livestock exposure to α-hexabromocyclododecane. (16th April 2022)

• Record Type: Journal Article
• Authors: Ratel, Jérémy; Mercier, Frédéric; Angénieux, Magaly; Kondjoyan, Nathalie; Abouelkaram, Saïd; Blinet, Patrick; Travel, Angélique; Royer, Eric; Baéza-Campone, Elisabeth; Cariou, Ronan; Jondreville, Catherine; Engel, Erwan
• Is Part Of: Food chemistry. Volume 374(2022)
• Publication Date: 2022-04-16
• Subjects: Volatolomics -- α-HBCDD -- Headspace-SPME -- GC–MS -- Livestock -- Food safety; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2021.131504
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: An experimental animal exposure to α-hexabromocyclododecane (HBCDD) was designed. Hens, broilers and pigs were exposed to realistic α-HBCDD doses in their diet. Liver volatolomics evidences exposure of the three farm animals to α-HBCDD. Most candidate markers are hydrocarbon, oxygenated, sulfur and aromatic compounds. Volatolomics, as an option for surveillance of chemical contamination in livestock. Abstract: Volatile organic compounds (VOC)-based metabolomics, or volatolomics, was investigated for revealing livestock exposure to chemical contamination. Three farm animals, namely laying hens, broilers, and pigs, were experimentally exposed to 5 or 50 ng α-HBCDD g −1 feed. Liver and egg yolk for hens were analysed by headspace-SPME-GC–MS to reveal candidate markers of the livestock exposure to α-HBCDD. For hens, 2-butanol was found as marker in egg. In liver, twelve VOCs were highlighted as markers, with three aromatic VOCs – styrene, o -xylene, α-methylstyrene – highlighted for the two α-HBCDD doses. For broilers, six markers were revealed, with interestingly, styrene and phenol which were also found as markers in hen liver. For pigs, ten markers were revealed and the seven tentatively identified markers were oxygenated and sulfur VOCs. The candidate markers tentatively identified were discussed in light of previous volatolomics data, in particular from a γ-HBCDD exposure of laying hens.