Evaluation of Cronobacter sakazakii biofilm formation after sdiA knockout in different osmotic pressure conditions. (January 2022)

• Record Type: Journal Article
• Authors: Cao, Yifang; Li, Li; Zhang, Yan; Liu, Fengsong; Xiao, Xinglong; Li, Xiaofeng; Yu, Yigang
• Is Part Of: Food research international. Volume 151(2022)
• Publication Date: 2022-01
• Subjects: Cronobacter sakazakii -- sdiA -- Biofilm -- Osmotic stress -- Motility -- Extracellular matrix; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2021.110886
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: The Δ sdiA formed more biofilms with extracellular matrix compared to the wild type. The motility of C. sakazakii was reduced in Δ sdiA compared to the wild type. C. sakazakii wild type and Δ sdiA formed stronger biofilm in TSB 3% NaCl. C. sakazakii wild type and Δ sdiA did not grown in TSB 12% NaCl. Abstract: This study characterizes the impact of sdiA on biofilm formation under normal or osmotic stress conditions in Cronobacter sakazakii by constructing a sdiA deletion mutant (Δ sdiA ). Here, the downregulation of flagellar assembly-related genes and upregulation of capsular, cellulose and lipopolysaccharide biosynthesis-associated genes in Δ sdiA were observed when compared to the wild type strain (WT) through transcriptomic analysis. Meanwhile, reduced ability of motility, enhanced cell surface hydrophobicity and stronger biofilms with extracellular matrix were observed in WT with deletion of sdiA . Both WT and Δ sdiA formed more biofilm in low osmotic stress medium, while in hyperosmolarity conditions, formation of biofilm was dramatically reduced. Our findings supported that sdiA might suppress biofilm formation of C. sakazakii by regulating biosynthesis of flagellar and extracellular polymeric substances. This study investigates the role of sdiA on biofilm formation in C. sakazakii, and provides the basis for the inhibition of C. sakazakii in food industry and infant-feeding.