Biosynthesis, structure and functionality of starch granules in maize inbred lines with different kernel dehydration rate. (30th January 2022)

• Record Type: Journal Article
• Authors: Qu, Jianzhou; Zhong, Yuyue; Ding, Li; Liu, Xingxun; Xu, Shutu; Guo, Dongwei; Blennow, Andreas; Xue, Jiquan
• Is Part Of: Food chemistry. Volume 368(2022)
• Publication Date: 2022-01-30
• Subjects: ACLall average chain lengths of the whole amylopectin analyzed by HPAEC -- ACLX average chain lengths (DP) of fraction X -- AGPase adenosine diphosphate glucose pyrophosphorylase -- AGPLS adenosine diphosphate glucose pyrophosphorylase large subunit -- AGPSS adenosine diphosphate glucose pyrophosphorylase small subunit -- AGS average granular size -- AUDDC area under the dry-down curve -- AP1 short amylopectin chains (DP 6-36) analyzed by GPC -- AP2 long amylopectin chains (DP 37-100) analyzed by GPC -- AM amylose chains (DP>100) analyzed by GPC -- DAP days after pollination -- DBE starch debranching enzyme -- DP degree of polymerization -- fa amylopectin chains with DP 6-12 analyzed by HPAEC -- fb1 amylopectin chains with DP 13-24 analyzed by HPAEC -- fb2 amylopectin chains with DP 25-36 analyzed by HPAEC -- fb3 amylopectin chains with DP >37 analyzed by HPAEC -- FPKM fragments per kilobase per million -- G′ storage modulus -- G″ loss modulus -- GBSS granule-bound starch synthase -- GPC gel-permeation chromatography -- ISA isoamylase -- KDR kernel dehydration rate -- KDW kernel dry weight -- KMC kernel moisture content -- MC moisture content -- PCA Principal Component Analysis -- PeakAM the position (mL) of amylose chains in GPC profiles -- PPi pyrophosphate -- PUL pullulanase -- RCX relative amount of fraction X -- RCAm amylose content -- SBE starch branching enzyme -- SS starch synthase -- SSS soluble starch synthase -- tan δ loss tangent -- TSC total starch content; Maize starch -- Molecular structure -- Gene expression -- Kernel dehydration rate; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2021.130796
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Relationships between maize dehydration rate and starch structure were disclosed. Dehydration rate was correlated with the expression of starch synthesis related genes. Maize with fast kernel dehydration rate had amylose with low molecular size. Maize with fast dehydration rate had low content of amylopectin chains of DP6-12. Maize with fast kernel dehydration rate had high retrogradation rate. Abstract: In this study, we report important relationships between kernel starch and kernel dehydration rate for eight maize inbred lines with different dehydration characteristics. High-throughput RNA sequencing data of starch biosynthesis-related genes showed that kernel moisture content and dehydration rate were both associated with differential expression of most starch biosynthetic genes. Especially, kernel moisture content was positively correlated with the increased expression of SBEI and SBEIIb, thereby potentially inducing biosynthesis of amylose with low molecular weight and amylopectin with low content of amylopectin chains with degree of polymerization (DP) 6-12 in inbred lines with fast kernel dehydration rate. We found a negative correlation between short amylopectin chains (DP 6-12) and the starch retrogradation rate. Hence, a low amount of amylopectin chains with DP 6-12 in the inbred lines with fast kernel dehydration rate was a plausible reason for their high short- and long-term retrogradation.