Novel insight into biological activity and phytochemical composition of Sorbus aucuparia L. fruits: Fractionated extracts as inhibitors of protein glycation and oxidative/nitrative damage of human plasma components. (September 2021)

• Record Type: Journal Article
• Authors: Rutkowska, Magdalena; Kolodziejczyk-Czepas, Joanna; Owczarek, Aleksandra; Zakrzewska, Anna; Magiera, Anna; Olszewska, Monika A.
• Is Part Of: Food research international. Volume 147(2021)
• Publication Date: 2021-09
• Subjects: Sorbus aucuparia -- Human plasma -- Protein glycation -- Protein nitration -- Lipid oxidation -- Polyphenols -- Free radicals; 3-NT 3-nitrotyrosine -- 3-NT-Fg 3-nitrotyrosine-containing fibrinogen -- AA ascorbic acid -- AE defatted acetone-water extract (1:1, v/v) -- AG aminoguanidine -- AGEs advanced glycation end products -- BF n-butanol fraction -- CFA caffeic acid -- CHA 5-O-caffeoylquinic acid -- CyE cyanidin chloride equivalents -- DEF diethyl ether fraction -- dw dry weight -- EAF ethyl acetate fraction -- FRAP ferric reducing antioxidant power -- GAE gallic acid equivalents -- HY quercetin 3-O-β-D-galactoside -- IQ quercetin 3-O-β-D-glucoside -- ME defatted methanol-water extract (1:1, v/v) -- NEAC non-enzymatic antioxidant capacity of plasma -- PB2 procyanidin B-2 -- PBMCs peripheral blood mononuclear cells -- PCA protocatechuic acid -- QU quercetin -- ROS reactive oxygen species -- SQ quercetin 3-O-β-sophoroside -- TBARS thiobarbituric acid-reactive substances -- TPA total proanthocyanidin content determined by n-butanol-HCl method -- TPC total phenolic content determined by Folin-Ciocalteu assay -- TPH total phenolic content determined by RP-HPLC-PDA as a sum of individual compounds -- WR water residue; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2021.110526
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Fifty-one phenolics were identified in the Sorbus aucuparia fruit extracts by LC-MS 3 . The extracts are rich in caffeoyl and feruloyl quinic and shikimic acids. The extracts considerably diminish ONOO − -induced changes in human plasma components. The extracts are potent antioxidants and inhibitors of protein glycation. The extracts are safe – do not influence the viability of human PBMCs. Abstract: Sorbus aucuparia L. is a source of edible fruits appreciated for their nutritional and medicinal properties. In this work some bioactivity mechanisms were evaluated, which might be connected with the traditional application of rowanberries in cardiovascular complications of diabetes. With the use of a panel of chemical and biological in vitro models the rowanberry extracts were proved to significantly inhibit the formation of advanced glycation end products, neutralise multiple oxidants generated in vivo, increase the non-enzymatic antioxidant capacity of human plasma and protect plasma components (proteins and lipids) against oxidative/nitrative damage at in vivo -relevant levels (1–5 µg/mL). Moreover, the extracts were found safe in cytotoxicity tests on the peripheral blood mononuclear cells. The comprehensive phytochemical profiling of the extracts (RP/HILIC-UHPLC-PDA-ESI-MS 3, HPLC-PDA, and UV-spectrophotometric methods) led to the identification of 51 phenolics, including caffeic and ferulic acids pseudodepsides (34 compounds, prevailing isomers of chlorogenic acid and cynarin, total content up to 269.4 mg/g), flavonols (mostly quercetin glycosides, up to 5.8 mg/g), flavan-3-ol derivatives (proanthocyanidin oligomers and polymers, up to 17.0 mg/g), and simple phenolic acids. The experiments on model constituents of the extracts and correlation studies were used to evaluate contribution of polyphenols to the observed effects. Taking into account the possible additive and synergistic effects, the co-occurrence of various compounds was indicated as partly responsible for biological activity of the fruits. Considering both the composition and activity parameters, the methanol–water (1:1, v/v ) extract and its concentrated phenolic fractions appeared to be the most advantageous for biological application.