In vitro colonic fermentation profiles and microbial responses of propionylated high-amylose maize starch by individual Bacteroides-dominated enterotype inocula. (June 2021)

• Record Type: Journal Article
• Authors: Xie, Zhuqing; Ding, Li; Huang, Qiang; Fu, Xiong; Liu, Feitong; Dhital, Sushil; Zhang, Bin
• Is Part Of: Food research international. Volume 144(2021)
• Publication Date: 2021-06
• Subjects: DS degree of substitution -- F/B Firmicutes to Bacteroidetes ratio -- FOS fructooligosaccharides -- GC-FID Gas Chromatography with Flame Ionisation Detector -- HAMS high-amylose maize starch -- Log(P/B) the log Prevotella to Bacteroides ratio -- NMDS non-metric dimensional scoring -- PCR polymerase chain reaction -- QIIME Quantitative Insights in the Microbial Ecology software package -- RS resistant starch -- SCFAs short-chain fatty acids; Propionylated high-amylose maize starch -- In vitro colonic fermentation -- Bacteroides-dominated enterotype -- Short-chain fatty acids; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2021.110317
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Individual Bacteroides -dominated enterotype did not influence gas production. Introduced propionyl groups could be released during fermentation. The relative abundance of Bacteroides was promoted for each individual. Gut microbiota changes were related to the initial microbiota composition. Abstract: The concept of "enterotype" has been proposed to differentiate the gut microbiota between individual humans, and different dominant bacteria utilize fiber substrates with different fermentation properties and microbial changes. In this study, we made propionylated high-amylose maize starch and investigated both in vitro fecal fermentation properties and microbial responses by individual Bacteroides -dominated enterotype inocula. Propionyl group substitution of HAMS did not significantly change gas production profiles, suggesting that the gas production during fermentation is independent of propionylation. The final concentration of released propionate significantly increased (10.26–12.60 mM) as a function of propionylation degree, suggesting that the introduced propionyl groups can increase the concentration of short-chain fatty acids (SCFA) during colonic fermentation. At the genus level, Bacteroides was obviously promoted for all donors with the final abundance in the range of 0.1–0.24, indicating that propionylated high-amylose maize starch changed the structure and abundance of microbiota compared to unmodified starch. Besides, the non-metric dimensional scoring (NMDS) plots showed that those changes were related to the initial microbiota composition. The results may offer useful information for the design of personalized food products and relevant therapies at least within Bacteroides -dominated enterotype.