Triterpenoids from functional mushroom Ganoderma resinaceum and the novel role of Resinacein S in enhancing the activity of brown/beige adipocytes. (October 2020)

• Record Type: Journal Article
• Authors: Huang, Yanjie; Wei, Gang; Peng, Xingrong; Hu, Guilin; Su, Haiguo; Liu, Junli; Chen, Xia; Qiu, Minghua
• Is Part Of: Food research international. Volume 136(2020)
• Publication Date: 2020-10
• Subjects: Ganoderma resinaceum -- Triterpenoids -- Brown adipocytes -- Obesity; BAT brown adipose tissue -- UCP1 uncoupling protein 1 -- PGC1α peroxisome proliferator-activated receptor coactivator alpha -- mtDNA mitochondrial DNA -- NRF nuclear respiratory factor -- Tfam mitochondrial transcription factor A -- COXII cyclooxygenase-2 -- PRDM16 PR domain containing 16 -- CPT1α carnitine palmitoyl transferase 1α -- Pparα peroxisome proliferator-activated receptor α -- Tbx1 T-box transcription factor 1 -- Tmem26 transmembrane protein 26 -- OCR oxygen consumption rates -- PPAR peroxisome proliferator-activated receptor -- AMPK adenosine 5ʹ-monophosphate-activated protein kinase -- HSL hormone-sensitive triglyceride lipase; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2020.109303
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Four new compounds (1 –4 ) and four known compounds (5 –8 ) were obtained from Ganoderma resinaceum . Resinacein S (Res S; 5 ) reduced lipid droplets size but not affect the differentiation of C3H10T1/2 cells. Res S increased the expression of brown and beige cell markers in differentiated C3H10T1/2 cells. Res S increased β -oxidation and pro-lipolytic activities in differentiated C3H10T1/2 cells. Res S induced mitochondrial biogenesis and activity in differentiated C3H10T1/2 cells. Abstract: As the major biologically active constituents in Ganoderma species, Ganoderma triterpenoids (GTs) also showed potential anti-obesity effect in recent reports. To further elucidate the anti-obesity effect of GTs, four new compounds Ganoderenses H–K (1 –4 ) and four known compounds (5 –8 ) from Ganoderma resinaceum were determined by extensive spectroscopic analysis. The absolute configurations of Ganoderenses H (1 ), I (2 ), and Resinacein S (Res S; 5 ) were confirmed for the first time by X-ray crystallographic analysis. Then the effects of these triterpenoids on brown/beige adipocytes were further analyzed in vitro . Our results may be summarized as follows: (1) Res S reduced lipid droplets size by regulating lipid metabolism, but not affect the differentiation of C3H10T1/2 cells. (2) Res S increased the expression of brown and beige adipocytes markers and enhanced the activity of brown and beige adipocytes (e.g., increased β -oxidation and pro-lipolytic activities et al.) in differentiated C3H10T1/2 cells. (3) Res S induced mitochondrial biogenesis and increased mitochondrial OCR in differentiated C3H10T1/2 cells. In conclusion, Res S is potential for activating the function of brown and beige adipocytes, thus having potential therapeutic implications for obesity and associated metabolic diseases.