Saccharum Granorum ameliorated mitochondrial dysfunction in chronic atrophic gastritis rats using organelle-specific metabolomics and network pharmacology approaches. (October 2020)

• Record Type: Journal Article
• Authors: Liu, Yuetao; Li, Xinqi; Qin, Xuemei
• Is Part Of: Food research international. Volume 136(2020)
• Publication Date: 2020-10
• Subjects: Saccharum Granorum -- Chronic atrophic gastritis -- Mitochondrial dysfunctions -- Metabolomics -- Network pharmacology; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2020.109503
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Abstract: Saccharum Granorum (YiTang) is a nutrient-rich food which could significantly treat chronic atrophic gastritis (CAG). However, its mechanism remains unclear. In the present work, a mitochondria-specific metabolomics approach based on ultra high performance liquid chromatography coupled with quadrupole - Exactive mass spectrometry (UHPLC-Q exactive MS) was carried out to evaluate its protection against energy dysfunction induced by CAG. Sixteen endogenous metabolites were firstly screened out to characterize the mitochondria dysfunction associated with CAG. Pretreatment of YiTang could significantly regulate these targeted metabolites involved into fatty acid β -oxidation pathway and amino acid metabolism. Network pharmacology further showed the upstream proteins related to these targeted metabolites exerted good affinities with ingredients of YiTang. These findings provided a unique perspective on localized metabolic regulation of YiTang in rats with CAG, which gave us new insights into the pathogenesis of CAG, and its targets for clinical diagnosis and treatment.