Ultra-highly sensitive organophosphorus biosensor based on chitosan/tin disulfide and British housefly acetylcholinesterase. (15th September 2020)

• Record Type: Journal Article
• Authors: Liu, Xinke; Sakthivel, Rajalakshmi; Liu, Wai-Ching; Huang, Ching-Wen; Li, Jian; Xu, Chuyu; Wu, Yueqi; Song, Lijun; He, Wei; Chung, Ren-Jei
• Is Part Of: Food chemistry. Volume 324(2020)
• Publication Date: 2020-09-15
• Subjects: British housefly acetylcholinesterase -- Chitosan -- Tin sulfide -- Cyclic voltammetry -- Organophosphorus pesticides; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2020.126889
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: An organophosphorus biosensor based on CHIT-SnS2 /AChE was proposed. The AChE/CHIT-SnS2 /GCE was applied for the detection of chlorpyrifos. The fabricated electrode shows the wide linear range with lowest LOD of 0.02 nM. The practicality of the biosensor was evaluated in cabbage and Malabar spinach. Abstract: Pesticides have been extensively applied worldwide to protect crops from worms and insects; however, the continuous use of pesticides affects ecosystems, agricultural product safety, nontarget organisms, and human health. In this paper, we report a highly sensitive biosensor for the determination of pesticides based on tin sulfide (SnS2 ) and chitosan (CHIT) nanocomposites decorated with a unique British housefly acetylcholinesterase (AChE). The hydrothermally synthesized nano-SnS2 mixed with chitosan solution (CHIT-SnS2 ) was drop-casted onto a glassy carbon electrode (GCE). Subsequently, the British housefly AChE was immobilized on the CHIT/SnS2 -coated GCE that was then employed for pesticide detection. The developed biosensor showed an ultra-high sensitivity and wide linear detection range from 0.02 nM to 20000 nM with a detection limit of 0.02 nM for the detection of chlorpyrifos as the model pesticide. Furthermore, the AChE/CHIT-SnS2 /GCE exhibited acceptable storage stability, good reproducibility, and selectivity.