Gastrointestinal digestion enhances the endothelium-dependent vasodilation of a whey hydrolysate in rat aortic rings. (July 2020)

• Record Type: Journal Article
• Authors: Ozorio, Luísa; Matsubara, Natália Kimie; da Silva-Santos, José Eduardo; Henry, Gwenaële; Le Gouar, Yann; Jardin, Julien; Mellinger-Silva, Caroline; Cabral, Lourdes M.C.; Dupont, Didier
• Is Part Of: Food research international. Volume 133(2020)
• Publication Date: 2020-07
• Subjects: Whey protein -- Peptides -- Hydrolysis -- Digestion -- Vascular relaxation -- Hypertension; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2020.109188
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: 198 peptides were generated during the digestion of the whey hydrolysate developed. The vasorelaxant potential of the whey peptides was enhanced after digestion. The digested whey hydrolysate presents an EC50 of 1.07 (0.75–1.51) µg·mL −1 . The digested vasorelaxant peptides act via endothelium-dependent pathways. Abstract: Whey proteins present encrypted biofunctional peptides that need to be released from the native protein to exert their biological activity. Antihypertensive whey peptides are the most studied ones, which can be explained by high prevalence of this chronic degenerative disease. The present study investigated whether the molecular changes occurred during the gastrointestinal digestion of a whey protein hydrolysate could modulate its vasorelaxant potential in rat aortic rings. Spectrophotometric data and SDS-PAGE gel showed a small degree of hydrolysis during the gastric phase and intense intestinal proteolysis. RP-HPLC revealed the formation of a large peptide profile. During the simulated digestion, 198 peptides were generated and identified and, left-shifted the concentration-response curve of the endothelium-dependent vasorelaxation, as recorded for the digested hydrolysates. In conclusion, gastrointestinal digestion of the whey hydrolysate leads to the generation of bioactive peptides with enhanced vasodilatory potency, reinforcing the relevance of whey-derived products in blood pressure regulation.