Evaluation of fruta-do-lobo (Solanum lycocarpum St. Hill) starch on the growth of probiotic strains. (July 2020)

• Record Type: Journal Article
• Authors: Pereira, Ana Paula Aparecida; Lauretti, Leonardo Borges Chatagnier; Alvarenga, Verônica Ortiz; Paulino, Bruno Nicolau; Angolini, Célio Fernando Figueiredo; Neri-Numa, Iramaia Angelica; Orlando, Eduardo Adilson; Pallone, Juliana Azevedo Lima; Sant'Ana, Anderson S.; Pastore, Glaucia Maria
• Is Part Of: Food research international. Volume 133(2020)
• Publication Date: 2020-07
• Subjects: Resistant starch -- Short-chain fatty acid -- Fruta-do-lobo -- Prebiotic; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2020.109187
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Fruta-do-lobo starch (FLS) contains 30% of resistant starch. For the first time we evaluated the potential prebiotic activity of FLS. Bifidobacterium and Lactobacillus were able to use FLS as a carbon source. FLS increased the acetate production. Abstract: Fruta-do-lobo ( Solanum lycocarpum St. Hill) is a native fruit commonly used in Brazilian folk medicine as a hypoglycemic agent. These properties are attributed to their starch, mainly its resistant fraction. Resistant starch has shown to increases the growth of Bifidobacterium and Lactobacillus in the gut, even though not being selective for these strains. In this scenario, this study aimed to investigate the potential prebiotic activity of fruta-do-lobo starch (FLS). FLS showed around 30% of resistant starch and their prebiotic potential was evaluated with five probiotic strains L. acidophilus (LA3 and LA5), L. casei (LC01) and B. animalis (BB12) and B. lactis (BLC1) in a concentration range of 1.0–2.0% of starch. In a preliminary screening, we evaluated, during 48 h, the viability of the starch with promoting growth agent. An increase in the growth of the probiotic strains tested was observed. We also evaluated the microorganism's metabolic activity by assessing the short-chain fatty acid (SCFA) production, using the best starch growth promotion conditions (2% of FLS and strains BLC1, LA5, and LC01). As expected, MRS and lactose were preferentially metabolized by BLC1, with the highest growth rates: 0.231 and 0.224 h −1, respectively. However, for this strain, the FLS growth rate (0.222 h −1 ) was 65% higher than FOS (0.144 h −1 ). Also, for LA5 FLS promoted higher growth (0.150 h −1 ) than FOS (0.135 h −1 ). Additionally, FLS promoted acetate production. These data are promising and indicate that FLS may have prebiotic potential and more studies need to be done with pathogenic microorganisms.