Workflow towards the generation of bioactive hydrolysates from porcine products by combining in silico and in vitro approaches. (June 2020)

• Record Type: Journal Article
• Authors: Bechaux, Julia; Ferraro, Vincenza; Sayd, Thierry; Chambon, Christophe; Le Page, Jean François; Drillet, Yoan; Gatellier, Philippe; Santé-Lhoutellier, Véronique
• Is Part Of: Food research international. Volume 132(2020)
• Publication Date: 2020-06
• Subjects: Biologically active peptides -- Enzymatic hydrolysis -- In silico studies -- Mass spectrometry -- Porcine offals; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2020.109123
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Porcine byproduct valorization allows the development of the circular economy. Bioactive peptides generation must combine in silico and in vitro methods. The use of the BIOPEP tool is relevant for in silico potentiality studies. Heart, liver, lung and muscle are source of DPP4i and antioxidant peptides. Identification of bioactive sequences by mass spectrometry needs improvements. Abstract: Food-derived bioactive peptides have generated an increasing interest in the field of health and well-being research. They can act either against the metabolic syndrome, participate in regulating the oxidation balance or act on the immune system. The aim of this study is to develop a workflow to generate bioactive peptides from three porcine offals namely, heart, liver, and lung and one muscle the Longissimus Dorsi, by combining in silico and in vitro approaches. Bioinformatics tools ( e.i. BIOPEP and Uniprot) permitted to orientate the choice of enzymes for generating abundant bioactive peptides from the four studied porcine products. With papain and subtilisin, the main bioactivities potentially released were ACE inhibitors, DPP4 inhibitors and antioxidant peptides. An in vitro validation study using papain and subtilisin demonstrated high DPP4 inhibitors and antioxidant bioactivities for the generation of peptides. This work allowed: i ) the identification of all proteins that composed porcine heart, liver, lung and LD muscle that could be useful for the scientific community, ii ) the development of a workflow to select most abundant proteins in a product while considering abundance factors and iii ) the potential of porcine meat and offals to generate DPP4 inhibitors and antioxidant peptides. However, there is still a need in developing new tools in order to face limitations of mass spectrometry for the identification of peptides with less than six amino acids. Such a work may contribute to the development of the circular economy and the innovative creation of value-added products from animal production.