Clitoria ternatea L. petal bioactive compounds display antioxidant, antihemolytic and antihypertensive effects, inhibit α-amylase and α-glucosidase activities and reduce human LDL cholesterol and DNA induced oxidation. (February 2020)

• Record Type: Journal Article
• Authors: Escher, Graziela Bragueto; Marques, Mariza Boscacci; do Carmo, Mariana Araújo Vieira; Azevedo, Luciana; Furtado, Marianna Miranda; Sant'Ana, Anderson S.; da Silva, Marcia Cristina; Genovese, Maria Inês; Wen, Mingchun; Zhang, Liang; Oh, Won Young; Shahidi, Fereidoon; Rosso, Neiva Deliberali; Granato, Daniel
• Is Part Of: Food research international. Volume 128(2020)
• Publication Date: 2020-02
• Subjects: Principal component analysis -- Response surface methodology -- Anthocyanins -- Unconventional foods -- Antihypertensive activity -- Inhibitor of DNA strand scission; Caffeic acid (PubChem CID: 689043) -- Delphinidin-3-O-glucoside (PubChem CID: 443650) -- 2, 4-Dihydroxybenzoic acid (PubChem CID: 1491) -- Gallic acid (PubChem CID: 370) -- p-Coumaric acid (PubChem CID: 637542) -- Procyanidin A2 (PubChem CID: 124025) -- Protocatechuic acid (PubChem CID: 72) -- Quercetin-3-rutinoside (PubChem CID: 5280805) -- Syringic acid (PubChem CID: 10742); ACE-I angiotensin-I-converting enzyme -- CCA Cu2+ chelating ability -- RCFC reducing capacity of the Folin-Ciocalteu reagent -- CLE crude lyophilized extract -- H2O2 hydrogen peroxide -- HPLC-PAD-UV high-performance liquid chromatography - photodiode array detector - fluorescence detector -- LDL low-density lipoprotein cholesterol -- NAF non-anthocyanic flavonoid -- NFP non-flavonoid phenolic -- ORAC oxygen radical absorption capacity -- PCA principal components analysis -- PPE partially purified extract -- ROS reactive oxygen species -- RSM response surface methodology -- TPC total phenolic content; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2019.108763
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Bioactive compounds of Clitoria ternatea petals were extracted at 40 °C for 30 min. Aqueous extract has in vitro antihemolytic and antihyperglycemic effects. Lyophilized extract inhibited 61% of angiotensin-I-converting enzyme in vitro. Extracts showed an inhibitory effect of DNA strand scission and LDL cholesterol oxidation. Intracellular antioxidant activity with concentrations above 100 μg/mL of extract. Abstract: The purpose of this study was to use a statistical approach to optimise the experimental conditions regarding the extraction of bioactive compounds, and to analyse the in vitro functional properties of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of Clitoria ternatea petals. The results showed that the factors of temperature and time influenced the extraction of phenolic compounds, antioxidant activity and the physicochemical parameters. Simultaneous optimisation showed that the same levels of bioactive compounds were extracted when using temperatures from 11.7 to 68.3 °C and times from 8.47 to 51.12 min. Principal component analysis revealed the experimental conditions that provided the extraction producing the highest level of phenolic content (40 °C/30 min). The CLE showed antimicrobial activity; protective effect against hemolysis of erythrocytes; inhibition of α-amylase, α-glucosidase and angiotensin-I-converting (ACE-I) enzymes; and inhibition of lipid peroxidation. The CLE and PPE demonstrated oxygen radical absorption capacity; inhibition of DNA strand scission; inhibition of LDL cholesterol oxidation; intracellular antioxidant activity against reactive oxygen species (>100 μg/mL); and no cytotoxicity (IC50, GI50 and LC50 > 900 μg/mL) against A549, HCT8 and IMR90 cell lines.