Carboxymethylpachymaran-zein coated plant microcapsules-based β-galactosidase encapsulation system for long-term effective delivery. (February 2020)

• Record Type: Journal Article
• Authors: Deng, Ziyu; Wang, Shishuai; Zhou, Bin; Li, Jing; Zhou, Peiyuan; Li, Bin; Liang, Hongshan
• Is Part Of: Food research international. Volume 128(2020)
• Publication Date: 2020-02
• Subjects: Enzyme activity -- Zein -- Carboxymethylpachymaran -- Sunflower sporopollenin exine capsules -- Stabilization -- Controlled release; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2019.108867
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: A green, safe and effective system to stabilize β-galactosidase was prepared. This system was assessed with high enzyme retention rate. This system impacted pH-responsive property. This system improved its performance in digestion of lactose. This system was for protein long-term protection and controlled release application. Abstract: Oral delivery of β-galactosidase (β-Gal) for alleviating lactose intolerance is a challenge due to its degradation in the harsh gastrointestinal tract (GIT). In this study, sunflower sporopollenin exine capsules (SECs)-based systems were fabricated to serve as protective microcapsules in order to improve the stability as well as to impact the pH-responsive release of β-Gal in GIT. The SECs extraction and loading process were optimized with the maximum residual activity of 82.75 ± 2.16%. Furthermore, the comparison of two systems indicated that β-Gal loaded into SECs which were entrapped in CMP-zein system was superior to that in zein system in terms of delivering β-Gal to the intestinal tract. Additionally, the interaction between CMP and zein confirmed by FTIR might contribute to the increased resistance to the GIT. Collectively, these results suggested that SECs-based CMP-zein system might be useful for encapsulation, protection, and delivery of bioactive substances.