A label-free shotgun proteomics analysis of macadamia nut. (March 2020)

• Record Type: Journal Article
• Authors: Rost, Johanna; Muralidharan, Sridevi; Lee, N. Alice
• Is Part Of: Food research international. Volume 129(2020)
• Publication Date: 2020-03
• Subjects: Macadamia nut soluble proteome -- Protein identification -- LC-MS/MS -- In-silico analysis -- Potential allergen -- Cross-reactivity -- AllergenOnline; MiAMP1 Macadamia antimicrobial peptide 1 -- MiAMP2 Macadamia antimicrobial peptide 2 -- IEDB-AR Immune Epitope Database Analysis Resource -- NCBI National Centre for Biotechnology Information -- MS/MS Tandem mass spectrometry -- LTQ-FT Linear ion Trap with a Fourier Transform ion cyclotron resonance mass spectrometer -- BLAST Basic Local Alignment Search Tool -- FARRP Food Allergy Research and Resource Program -- GO Gene Ontology; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2019.108838
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Cascading of the amino acid and carbohydrate metabolic pathways in macadamia nut are proposed for the first time. The defense response proteins are the most abundant group of proteins in macadamia nut. The in silico analysis revealed conglutins and vicilins with potential allergenicity. Abstract: In this study, we present a systematic proteomic overview of macadamia nut using a label-free shotgun proteomic approach. We identified 947 proteins in 723 clusters and gene ontology analysis revealed proteins across 46 functional categories including carbohydrate metabolism (10%), protein metabolic processes (5%), amino acid metabolism (4%), transport (4%), stress response (3%), lipid metabolism (3%), protein folding (3%) and defense response (1.4%). The defense response proteins accounted for 24% of the total peptide abundance. The vicilin-like macadamia antimicrobial peptides 2–3 (MiAMP2) was the most abundant protein, followed by glyceraldehyde-3-phosphate dehydrogenase 3, 11S legumin-like protein, 2-phospho-D-glycerate hydrolase and heat shock 70 kDa protein among others. The cascading of amino acid and carbohydrate metabolic pathways in macadamia nut were constructed against reference maps from KEGG and proposed for the first time. Results were also indicative of useful protein candidates with possible allergenic potential and cross-reactivity in macadamia nut. The in-silico analysis revealed homology and linear epitope similarities to known allergens such as conglutin β allergen from lupin, Jug r2 vicilin allergens from walnut, Ara h3 11S globulin from peanut, small rubber particle protein Hev b3, hevein, enolase 2, HSP 70kDa Cla h4, Der f28 allergen, and methylglyoxalases. Label-free shotgun proteomics reveal valuable insights into the genetic and biological makeup of macadamia nut proteome and provide guidance on protein candidates with allergenic potential for further immunological investigation. Data are available via ProteomeXchange with identifier PXD015364.