Improved recovery of galloyl metabolites from mango (Mangifera indica L.) in human plasma using protein precipitation with sodium dodecyl sulfate and methanol. (March 2020)

• Record Type: Journal Article
• Authors: Barnes, Ryan C.; Kim, Hyemee; Mertens-Talcott, Susanne U.; Talcott, Stephen T.
• Is Part Of: Food research international. Volume 129(2020)
• Publication Date: 2020-03
• Subjects: Mango -- Gallic acid -- Sodium dodecyl sulfate -- Metabolite -- Pharmacokinetics; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2019.108812
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: SDS as an extraction aide enhanced recovery of gallic acid metabolites from plasma. (n = 10) were fed 400 g of mango and metabolites were quantified in plasma over 8 h. 5 gallic acid derived metabolites were quantified in plasma with the SDS-MeOH method. Abstract: Extraction of polyphenolic metabolites from blood fractions can be challenging since compound recovery can be limited by chemical structure, polarity, and protein-binding affinity of analytes. Gallic acid and its metabolites exhibit particularly low recoveries from plasma and can lead to an underestimation of their bioavailability from foods. A modified method to extract free gallic acid and its metabolites from human plasma aided by sodium dodecyl sulfate and acidified methanol (SDS-MeOH) was applied to extract free gallic acid and its metabolites from human plasma after a single consumption of 400 g of mango (cv. Ataulfo) pulp by 10 healthy male and female subjects. The use of SDS-MeOH facilitated extraction of significantly (p < 0.05) more pyrogallol, free gallic acid, 4- O -methylgallic acid, and ethyl gallate with recovery rates exceeding 80% in standard recovery from human blood plasma when compared to conventional methods that rely on solvent extraction or solid phase extraction. The method was reproducible and precise for standards from 50 to 500 μg/L. In pharmacokinetic plasma samples five predominant metabolites of gallic acid were tentatively characterized by HPLC-MS and absorption kinetics evaluated over 8 h for catechol- O -sulfate, 4- O -methylgallic acid-3- O -sulfate, and pyrogallol- O -sulfate, methylpyrogallol- O -sulfate, and 4- O -methylgallic acid with AUC0-8h of 9520 ± 3370, 6030 ± 1310, 5990 ± 1690, 4020 ± 1040, and 2790 ± 1190 μg/L h respectively. Plasma extraction was rapid and reproducible with superior recovery rates compared to conventional methods when evaluating polar phenolic metabolites.