Effects of gastrointestinal digested polyphenolic enriched extracts of Chilean currants (Ribes magellanicum and Ribes punctatum) on in vitro fecal microbiota. (March 2020)

• Record Type: Journal Article
• Authors: Burgos-Edwards, Alberto; Fernández-Romero, Arantxa; Carmona, Manuel; Thuissard-Vasallo, Israel; Schmeda-Hirschmann, Guillermo; Larrosa, Mar
• Is Part Of: Food research international. Volume 129(2020)
• Publication Date: 2020-03
• Subjects: Bifidobacterium spp. -- Faecalibacterium prausnitzii -- Akkermansia muciniphila -- Lactobacillus spp. -- Clostridium cluster XIVa -- Ribes spp. -- In vitro fermentation; BCFA branched-chain fatty acids -- 3-CQA 3-caffeoylquinic acid -- 5-CQA 5-caffeoylquinic acid -- FOS fructooligosaccharides -- GID gastrointestinal digestion -- HCA hydroxycinnamic acids -- IBD inflammatory bowel diseases -- ID-PEE intestinal digested polyphenol-enriched extract -- PEE polyphenol-enriched extract -- SCFA short-chain fatty acids; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2019.108848
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Chilean Ribes polyphenols were digested and tested in an in vitro model of gut microbiota. Digested Chilean currants reduced branched chain fatty acids and SCFA throughout fermentation. Both digested Ribes promoted the growth of Clostridium cluster XIVa. Ribes punctatum increased Faecalibacterium prausnitzii population. Abstract: Chilean currants ( Ribes magellanicum and Ribes punctatum ) are wild polyphenol-rich berries with interesting bioactivities in several in vitro models. The aim of this study was to investigate the effects of the pre-digested PEE (polyphenol-enriched extract) in a simulated colon model. Fruits were extracted, submitted to simulated gastrointestinal digestion and further colonic fermentation with feces from healthy human donors. Samples were taken at 1, 4, 8 and 24 h of incubation, monitoring pH, ammonia, branched-chain fatty acids (BCFA), short-chain fatty acids (SCFA) and bacterial growth. FOS (fructooligosaccharides) and fecal slurry without treatments were positive and negative control, respectively. Both Ribes species reduced (p < 0.05) both BCFA and SCFA at 24 h. R. punctatum promoted the growth (p < 0.05) of beneficial bacteria such as Clostridium cluster XIVa, and Faecalibacterium prausnitzii ; while a trend to increase Akkermansia muciniphila was observed. R. magellanicum increased (p < 0.05) Clostridium cluster XIVa population. Escherichia coli, Lactobacillus spp. and Bifidobacterium spp. remained unaffected. Our results suggest that polyphenols from R. punctatum and R. magellanicum may modulate both bacterial metabolism and some selected gut beneficial bacteria under simulated conditions. Therefore, Chilean currants might be useful as supplements to maintain a healthy colon; however, further in vivo studies are needed to confirm their effect and their mechanisms.