HPLC-DAD-APCI-MSn analysis of the genuine carotenoid pattern of pineapple (Ananas comosus [L.] Merr.) infructescence. (January 2020)

• Record Type: Journal Article
• Authors: Steingass, Christof B.; Vollmer, Kathrin; Lux, Peter E.; Dell, Carolin; Carle, Reinhold; Schweiggert, Ralf M.
• Is Part Of: Food research international. Volume 127(2020)
• Publication Date: 2020-01
• Subjects: Carotenoids -- Xanthophyll esters -- Tropical fruit; APCI atmospheric pressure chemical ionisation -- BHA butylated hydroxyanisole -- BHT butylated hydroxytoluene -- CID collision induced dissociation -- dah days after harvest -- FW fresh weight -- HPLC-DAD high performance liquid chromatography-diode array detection -- λmax absorption maxima -- LP light petroleum -- MSn multiple-stage mass spectrometry -- RAE retinol activity equivalent -- tBME tert-butyl methyl ether -- tR retention time; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2019.108709
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Detailed HPLC-DAD-APCI-MS n analysis of pineapple carotenoids. Identification of 21 lipophilic pigments in the pineapple infructescence. Xanthophyll esters are reported for the first time as pineapple constituents. Quantitation of carotenoids in four varieties cultivated in Ghana. Abstract: The genuine carotenoid pattern of pineapple infructescence was assessed by HPLC-DAD-APCI-MS n analysis. Prevailing pigments in the shell of 'MD2' (syn. "Extra Sweet") fruit were (all- E )-lutein and (all- E )-β-carotene, in addition to chlorophylls a and b . The edible flesh contained (all- E )-violaxanthin, (all- E )-β-carotene, and diverse esters of (9 Z )-violaxanthin with caprylic, capric, lauric, and myristic acid. The latter esters have been reported for the first time as pineapple constituents. Total carotenoid concentrations in the edible fractions of the four varieties 'Sugar Loaf', 'Smooth Cayenne', 'MD2', and 'Queen Victoria' cultivated in Ghana ranged between 29 and 565 µg/100 g of fresh weight (FW). Total carotenoids in the flesh of fully ripe 'MD2' fruit exported by air freight amounted to 302 µg/100 g of FW, those in green ripe samples dispatched by sea freight to 359–432 µg/100 g of FW. All yellow fleshed cultivars exhibited a highly similar qualitative carotenoid profile.