Lupine protein hydrolysates decrease the inflammatory response and improve the oxidative status in human peripheral lymphocytes. (December 2019)

• Record Type: Journal Article
• Authors: Cruz-Chamorro, Ivan; Álvarez-Sánchez, Nuria; Millán-Linares, María del Carmen; Yust, María del Mar; Pedroche, Justo; Millán, Francisco; Lardone, Patricia Judith; Carrera-Sánchez, Cecilio; Guerrero, Juan Miguel; Carrillo-Vico, Antonio
• Is Part Of: Food research international. Volume 126(2019)
• Publication Date: 2019-12
• Subjects: Lupine peptides -- Vegetable hydrolysates -- Bioactivity -- Peripheral blood mononuclear cells -- Cytokines -- Th1 response -- Inflammation -- Anti-oxidant; CAT catalase -- GPx glutathione peroxidase -- GR glutathione reductase -- GSH glutathione -- GSSG oxidized form of glutathione -- IL interleukin -- LPHs lupine protein hydrolysates -- PBMCs peripheral blood mononuclear cells -- PHA phytohaemagglutinin -- ROS reactive oxygen species -- SOD superoxide dismutase -- TAC total antioxidant capacity.; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2019.108585
• Languages: English
• ISSNs: 0963-9969

Abstract

Abstract: Although cell-free systems and immortalized cell lines have been used to demonstrate the potential health benefits of lupine proteins and peptides, no study has examined the effects of lupine protein hydrolysates (LPHs) on the immune and oxidative responses of non-immortalized human cells. Therefore, the aims of this study were to evaluate the effects of the in vitro administration of LPHs from Lupinus angustifolius on the immunological and oxidative statuses of human peripheral blood mononuclear cells (PBMCs) from 53 healthy donors. LPHs reduced PBMCs proliferation and the levels of Th1, Th9 and Th17 pro-inflammatory cytokines without being cytotoxic. LPHs also skewed the pro-/anti-inflammatory balance towards a Th2 protective response. Additionally, LPHs increased superoxide dismutase and catalase activities, and the total antioxidant capacity (TAC). This study is the first to show that LPHs reduce T cell inflammatory responses and improve the anti-inflammatory/pro-inflammatory cytokine balance and the TAC by PBMCs. Thus, LPHs may represent an effective option for developing nutritional strategies to prevent pathologies with underlying inflammation and oxidative stress. Graphical abstract: Unlabelled Image Highlights: LPHs reduce human PBMCs proliferation. Th1, Th9 and Th17 inflammatory responses are decreased in LPHs treated PBMCs. LPHs skew the pro-/anti-inflammatory balance towards a protective phenotype. The total antioxidant capacity is improved in LPHs-treated PBMCs.