Anthocyanins from black peanut skin protect against UV-B induced keratinocyte cell and skin oxidative damage through activating Nrf 2 signaling. Issue 10 (2nd October 2019)

• Record Type: Journal Article
• Authors: Li, Kaikai; Zhang, Mengying; Chen, Haoze; Peng, Jinming; Jiang, Fan; Shi, Xin; Bai, Yilin; Jian, Min; Jia, Yangyang
• Is Part Of: Food & function. Volume 10:Issue 10(2019)
• Page Start: 6815
• Page End: 6828
• Publication Date: 2019-10-02
• Subjects: Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Nutrition -- Periodicals; 664.07
• DOI: 10.1039/c9fo00706g
• Languages: English
• ISSNs: 2042-6496

Abstract

Abstract : Anthocyanins from black peanut skin could improve the UV-B induced skin damage mainly through the MAPK/Nrf2/HO-1- NQO1-GCLC signaling axis. Abstract : Excessive Ultraviolet (UV) irradiation induces skin damage. In the present study, the potential protective activity of anthocyanins (cyanidin-3- O -sophoroside and cyanidin-3- O -sambubioside) from black peanut against skin damage induced by UV-B was evaluated in vitro and in vivo . Treatment with anthocyanins significantly reversed UV-B induced oxidative damage and following apoptotic death in human HaCaT cells. Nuclear-factor-E2-related factor 2 (Nrf 2) was activated by anthocyanins through Nrf 2 protein stabilization and nuclear translocation, along with the expressions of antioxidant responsive element (ARE)- related genes (HO1, GCLC and NOQ1). Nrf 2 knockdown in HaCaT cells by targeted-shRNA plasmid markedly abolished the protective activity of anthocyanins against UV-B irradiation. Additionally, topical application of anthocyanins (5 mg cm −2 ) inhibited UV-B induced oxidative stress and cell apoptosis in BALB/c mouse skin tissues. The protective effect of anthocyanins can be explained by the regulation of oxidative-stress and the suppression of cell apoptosis through the activation of Nrf-2 by interaction with the MAPK and NF-κB signaling pathways. Our results suggested that anthocyanins from black peanut skin might be used as a potential photochemo-protective agent against UV-B induced skin damage.