Influence of in vitro digestion process on polyphenolic profile of skin grape (cv. Italia) and on antioxidant activity in basal or stressed conditions of human intestinal cell line (HT-29). (April 2018)

• Record Type: Journal Article
• Authors: Garbetta, A.; Nicassio, L.; D'Antuono, I.; Cardinali, A.; Linsalata, V.; Attolico, G.; Minervini, F.
• Is Part Of: Food research international. Volume 106(2018)
• Page Start: 878
• Page End: 884
• Publication Date: 2018-04
• Subjects: CAA cellular antioxidant activity -- CMHP cumene hydroperoxide -- DCFH-DA 2′, 7′-dichlorofluorescein diacetate -- FW fresh weight -- GI gastro-intestinal -- GSH reduced glutathione -- MCB monochlorobimane -- ROS Reactive Oxygen Species -- TPI total polyphenols identified by HPLC; Grape -- Polyphenols -- Digestion process -- Intestinal cell model -- Antioxidant activity -- Intracellular ROS -- Intracellular GSH; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2018.01.072
• Languages: English
• ISSNs: 0963-9969

Abstract

Abstract: White table grape cv. Italia is a typical component of the Mediterranean diet and a source of phenolic compounds, particularly abundant in the skin portion. The aim of this study was to characterize the phenolic profile of the table grape skin and to assess its stability after the in vitro digestion process. The main phenolic compounds identified by the HPLC-DAD analysis were: procyanidin B1, caftaric acid, catechin, coutaric acid, quercetin 3-glucuronide and quercetin 3-glucoside. All compounds showed a good stability after in vitro digestion (from 43 to 80%). Moreover, the influence of grape skin polyphenols on the modulation of ROS and GSH levels was evaluated in basal and in stressed conditions on human intestinal cells (HT-29). In basal conditions, a higher polyphenol concentrations exerted pro-oxidant effect corresponding to high ROS level and low GSH content. This effect was probably due to the polyphenolic oxidation in cell culture condition with consequent production of hydrogen peroxide. Otherwise, in stressed conditions, grape skin polyphenols exerted antioxidant effects up to 1.3 × 10 −6 μg/g and restored the stress-related GSH reduction. The in vitro digestion process attenuated the biological effect of grape skin polyphenols on intestinal cell line (HT-29). In conclusion, grape skin polyphenols showed different behavior in relation to their concentrations and to the intracellular ROS levels. Graphical abstract: Unlabelled Image Highlights: Grape skin polyphenols pattern did not change after in vitro digestion process. ROS levels in intestinal cells modulated bioactivity of polyphenols grape skin. Production of hydrogen peroxide by polyphenols in neutral environment.