1H NMR-based metabolomic profiling for identification of metabolites in Capsicum annuum cv. mirasol infected by beet mild curly top virus (BMCTV). (April 2018)

• Record Type: Journal Article
• Authors: Villa-Ruano, Nemesio; Velásquez-Valle, Rodolfo; Zepeda-Vallejo, L. Gerardo; Pérez-Hernández, Nury; Velázquez-Ponce, Manuel; Arcos-Adame, Victor M.; Becerra-Martínez, Elvia
• Is Part Of: Food research international. Volume 106(2018)
• Page Start: 870
• Page End: 877
• Publication Date: 2018-04
• Subjects: Capsicum annuum -- Beet mild curly top virus -- Metabolomic profiling -- 1H NMR -- Principal component analysis; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2018.01.065
• Languages: English
• ISSNs: 0963-9969

Abstract

Abstract: Beet mild curly top virus (BMCTV) is associated with an outbreak of curly top in chili pepper, tomato and other Solanaceae species, which can cause severe crop losses. The aim of this work was to obtain the 1 H NMR metabolomic profiling of both healthy chili peppers (cv. mirasol) and infected chili peppers with BMCTV in order to find chemical markers associated to the infection process. Significant differences were found between the two groups, according to principal component analysis and orthogonal projections to latent structure discriminant analysis. Compared to the asymptomatic peppers, the symptomatic fruits had higher relative abundance of fructose, isoleucine, histidine, phenylalanine and tryptophan. Contrarily, the asymptomatic samples showed greater amounts of malonate and isobutyrate. These results suggest that in diseased chili peppers there are metabolic changes related to the viral acquisition of energy for replication and capsid assembly. This is the first study describing the chemical profiling of a polar extract obtained from Capsicum annuum infected by BMCTV under open field conditions. Graphical abstract: Unlabelled Image Highlights: The 1 H NMR profiling of Capsicum annuum cv. mirasol is reported for the first time. Metabolic changes were observed during Beet Mild Curly Top Virus infection. Metabolic alterations suggest that sugar and amino acid pathways were affected. A reliable 1 H NMR method to compare the metabolic profiling of healthy and diseased chili peppers is shown.