Lupine allergen detecting capability and cross-reactivity of related legumes by ELISA. (1st August 2018)

• Record Type: Journal Article
• Authors: Koeberl, Martina; Sharp, Michael F.; Tian, Rongkai; Buddhadasa, Saman; Clarke, Dean; Roberts, James
• Is Part Of: Food chemistry. Volume 256(2018)
• Page Start: 105
• Page End: 112
• Publication Date: 2018-08-01
• Subjects: Ara h 1 (Ara h 2, Ara h 3, Ara h 5, Ara h 6, Ara h 7, Ara h 8, Ara h 9, Ara h 10, Ara h 11, and Ara h 14) Registered peanut (Arachis hypogaea) allergen with IUIS -- CV cultivated variety -- DF dilution factor -- ELISA enzyme-linked immunosorbent assay -- Gly m 1 (Gly m, Gly m 4, Gly m 5, Gly m 6, Gly m 7 and Gly m 8) Registered soya (Glycine max) allergen with IUIS -- IgE immunoglobulin E -- IUIS World Health Organization and International Union of Immunological Societies (WHO/IUIS) Allergen Nomenclature Sub-committee -- Len c 1 (Len c 3) Registered lentil (Lens culinaris) allergen with IUIS -- LOD limit of detection -- LOQ limit of quantification -- Lup an 1 registered blue lupine (Lupinus angustifolius) allergen with IUIS -- nsLTP non-specific lipid transfer proteins -- Pha v 3 Registered green bean (Phaseolus vulgaris) allergen with IUIS -- Pis s 1 (Pis s 2, Pis s 3) Registered pea (Pisum sativum) allergen with IUIS -- PR-10 pathogenesis-related Protein-10 -- SD standard deviation -- Vir g 1 (Vir g 2, Vir g 4, Vir g 6) Registered mung bean (Vigna radiata) allergen with IUIS; Lupinus angustifolius (Blue Lupine or Australian Sweet Lupine) -- Lupinus albus (White Lupine) -- Lupinus luteus (Yellow Lupine) -- ELISA test kit comparison -- Lupine cross-reactivity; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2018.02.043
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Comparison of enzyme-linked immunosorbent assays for lupine allergen detection. Determination of detection capability for three different lupine species. Investigation of lupine cross-reactivity to related legumes. Abstract: Lupine belongs to the genus Lupinus and includes three species commonly consumed by humans. The Lupinus genus is closely related to other legumes, such as peanuts, soya, chickpeas, peas, lentils and beans. However, the consumption of lupine (and related legumes) can cause severe allergenic reactions. Therefore, reliable analytical detection methods are required for the analysis of food samples. In this study three commercially available ELISA test kits were analyzed for the detection capability of three common lupine species, as well as cross-reactivity to related legumes. All three ELISA test kits could detect the lupine species, though with different sensitivities. Cross-reactivity varied for the ELISA test kits and all showed some cross-reactivity to related legume samples analyzed.