Identification of phenolic metabolites in human urine after the intake of a functional food made from grape extract by a high resolution LTQ-Orbitrap-MS approach. (October 2017)

• Record Type: Journal Article
• Authors: Sasot, Gemma; Martínez-Huélamo, Miriam; Vallverdú-Queralt, Anna; Mercader-Martí, Mercè; Estruch, Ramon; Lamuela-Raventós, Rosa M.
• Is Part Of: Food research international. Volume 100:Part 3(2017)
• Page Start: 435
• Page End: 444
• Publication Date: 2017-10
• Subjects: Grape pomace -- Human urine -- LTQ-Orbitrap -- High resolution mass spectrometry -- Polyphenols -- Identification -- Polyphenol metabolites; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2017.01.020
• Languages: English
• ISSNs: 0963-9969

Abstract

Abstract: Grape pomace (GP) is known to be a rich source of polyphenols with biological activity which may be used as functional ingredients for the development of new health-promoting products. Numerous studies have reported that bioactive compounds may act through multiple mechanisms. In order to verify the oral absorption and metabolism of grape polyphenols, we performed a prospective, randomised and cross-over acute study in 12 volunteers with two interventions: 500 mL of a functional beverage enriched with 200 mL of GP and 500 mL of a control beverage without GP. In this work, liquid chromatography coupled with an electrospray ionization hybrid linear ion trap quadrupole-Orbitrap-mass spectrometry (LC/ESI-LTQ-Orbitrap-MS) technique has been used to accurately identify phenolics in GP and human urine. In GP, 41 phenolic compounds were identified mainly procyanidins, phenolic acids and flavonols, and in human urine over 70 metabolites of phenolic compounds including microbiota metabolites, glucuronides and sulfate derivatives were detected. Overall, high resolution mass spectrometry (HR-MS) enhances the identification of a large variety of polyphenols and their metabolites with great mass accuracies for all molecular ions. Graphical abstract: Highlights: HPLC-LTQ-Orbitrap-MS allows the identification of the polyphenols in food and their derived metabolites in urine. This technique is crucial for elucidating compounds with a wide range of mass in complex matrices. LTQ-Orbitrap-MS is a suitable analyser for its combinations of high sensitivity, high resolution and high mass accuracy.