Influence of herd diet on the metabolome of Maasdam cheeses. (September 2019)

• Record Type: Journal Article
• Authors: Panthi, Ram R.; Sundekilde, Ulrik K.; Kelly, Alan L.; Hennessy, Deirdre; Kilcawley, Kieran N.; Mannion, David T.; Fenelon, Mark A.; Sheehan, Jeremiah J.
• Is Part Of: Food research international. Volume 123(2019)
• Page Start: 722
• Page End: 731
• Publication Date: 2019-09
• Subjects: Herd diet -- Feeding system -- Maasdam cheese -- Cheese metabolic profile -- 1H NMR -- 1H HRMAS NMR -- Cheese volatile compounds; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2019.05.026
• Languages: English
• ISSNs: 0963-9969

Abstract

Abstract: The untargeted metabolic profiles of ripened Maasdam cheese samples prepared from milk derived from three herd groups, fed: (1) indoors on total mixed ration (TMR), or outdoors on (2) grass only pasture (GRA) or (3) grass and white clover pasture (CLO) were studied using high resolution nuclear magnetic resonance ( 1 H NMR), high resolution magic angle spinning nuclear magnetic resonance ( 1 H HRMAS NMR) and headspace (HS) gas chromatography mass spectrometry (GC-MS). A total of 31 compounds were identified using 1 H NMR and 32 volatile compounds including 7 acids, 5 esters, 4 alcohols, 4 ketones, 4 sulfur compounds, 2 aldehydes, 3 hydrocarbons, 2 terpenes and a lactone were identified using GC–MS in Maasdam cheeses ripened for 97-d. On comparing the 1 H NMR metabolic profiles, TMR-derived cheese had higher levels of citrate compared to GRA-derived cheese. The toluene content of cheese was significantly higher in GRA or CLO compared to TMR cheeses and dimethyl sulfide was identified only in CLO-derived cheese samples as detected using HS GC–MS. These compounds are proposed as indicator compounds for Maasdam cheese derived from pasture-fed milk. Clear differences between outdoor or indoor feeding systems in terms of cheese metabolites were detected in the lipid phase, as indicated by principal component analysis (PCA) from 1 H HRMAS NMR spectra, although differences based on PCA of all 1 H NMR spectra and HS-GC–MS were less clear. Overall, this study presented the metabolite profile and identified specific compounds which may be useful for discriminating between ripened Maasdam cheese and related cheese varieties manufactured from indoor or outdoor herd-feeding systems. Graphical abstract: Unlabelled Image Highlights: Milk of indoor-fed cows led to higher levels of citrate in cheese. Milk of pasture-based cows led to higher levels of toluene in cheese. Feeding white clover to cows increased dimethyl sulfide levels in cheese.