Soluble extracts from carioca beans (Phaseolus vulgaris L.) affect the gut microbiota and iron related brush border membrane protein expression in vivo (Gallus gallus). (September 2019)

• Record Type: Journal Article
• Authors: Dias, Desirrê Morais; Kolba, Nikolai; Hart, Jon J.; Ma, Michelle; Sha, Sybil T.; Lakshmanan, Naveena; Nutti, Marilia Regini; Martino, Hércia Stampini Duarte; Glahn, Raymond P.; Tako, Elad
• Is Part Of: Food research international. Volume 123(2019)
• Page Start: 172
• Page End: 180
• Publication Date: 2019-09
• Subjects: Biofortification -- Dietary fiber -- Common beans -- Iron -- Brush border membrane -- Microbial populations; Fe Iron -- BBM Brush Border Membrane -- DMT1 Divalent metal transporter 1 -- AP Amino peptidase -- SI Sucrose isomaltase -- FPN Ferroportin -- ZnT1 zinc transporter 1; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2019.04.060
• Languages: English
• ISSNs: 0963-9969

Abstract

Abstract: The effect of soluble extracts with putative prebiotic ability extracted from various bean varieties on the intestinal brush border membrane (BBM) iron related proteins, and intestinal bacterial populations were evaluated using the Gallus gallus model and by the intra-amniotic administration procedure. Eight treatment groups [(non-injected; 18 MΩ H2 O; 40 mg/mL Inulin; 50 mg/mL BRS Perola (carioca standard); 50 mg/mL BRS Cometa (carioca, Fe biofortified); 50 mg/mL BRS Esteio (black, standard); 50 mg/mL SMN 39 (black, Fe biofortified); 50 mg/mL BRS Artico (white, standard)] were utilized. Tested groups reduced the relative abundance of Clostridium and E. coli compared to the Inulin group (positive control) and they did not affect the relative abundance of Bifidobacterium and Lactobacillus compared to the negative control (18MΩ H2 O). The relative expression of zinc transporter 1, ferroportin and amino peptidase were up-regulated in the BRS Cometa group (Fe-biofortified carioca beans). Results suggest that soluble extracts from carioca beans may improve the iron bioavailability by affecting intestinal bacterial populations, and BBM functionality. Graphical abstract: Unlabelled Image Highlights: Prebiotics from carioca beans increased the abundance of beneficial bacteria. Prebiotics from carioca beans limited the amount of potentially pathogenic bacteria. Prebiotics from carioca beans improved the intestinal function. Prebiotics from carioca beans may contribute to the Fe (and zinc) transport.