New insights into the chemical profiling, cytotoxicity and bioactivity of four Bunium species. (September 2019)

• Record Type: Journal Article
• Authors: Zengin, Gokhan; Paksoy, Mehmet Yavuz; Aumeeruddy, Muhammad Zakariyyah; Glamocilja, Jasmina; Sokovic, Marina; Diuzheva, Alina; Jekő, József; Cziáky, Zoltán; Rodrigues, Maria João; Custodio, Luisa; Mahomoodally, Mohamad Fawzi
• Is Part Of: Food research international. Volume 123(2019)
• Page Start: 414
• Page End: 424
• Publication Date: 2019-09
• Subjects: Bunium -- phytoconstituents -- cytotoxicity -- antioxidant -- enzyme inhibitors; ABTS 2, 2′-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid -- ACAE acarbose equivalent -- AChE acetylcholinesterase -- BChE butyrylcholinesterase -- CUPRAC cupric reducing antioxidant capacity -- DPPH 1, 1-diphenyl-2-picrylhydrazyl -- EDTAE EDTA equivalent -- FRAP ferric reducing antioxidant power -- GAE gallic acid equivalent -- GALAE galatamine equivalent -- HEK human embryonic kidney -- KAE kojic acid equivalent -- MBC minimum bactericidal concentration -- MIC minimum inhibitory concentration -- RE rutin equivalent -- TE trolox equivalent -- TPC Total phenolic content -- TFC Total flavonoid content; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2019.05.013
• Languages: English
• ISSNs: 0963-9969

Abstract

Abstract: Bunium species have been reported to be used both as food and in traditional medicines. The scientific community has attempted to probe into the pharmacological and chemical profiles of this genus. Nonetheless, many species have not been investigated fully to date. In this study, we determined the phenolic components, antimicrobial, antioxidant, and enzyme inhibitory activities of aerial parts of four Bunium species ( B. sayai, B. pinnatifolium, B. brachyactis and B. macrocarpum ). Results showed that B. microcarpum and B. pinnatifolium were strong antioxidants as evidenced in the DPPH, ABTS, CUPRAC, and FRAP assays. B. brachyactis was the most effective metal chelator, and displayed high enzyme inhibition against cholinesterase, tyrosinase, amylase, glucosidase, and lipase. The four species showed varied antimicrobial activity against each microorganism. Overall, they showed high activity against P. mirabilis and E. coli (MIC and MBC <1 mg mL −1 ). B. brachyactis was more effective against Aspergillus versicolor compared to the standard drug ketoconazole. B. brachyactis was also more effective than both ketoconazole and bifonazole against Trichoderma viride . B. sayai was more effective than ketoconazole in inhibiting A. fumigatus . B. sayai was most non-toxic to HEK 293 (cellular viability = 117%) and HepG2 (cellular viability = 104%). The highest level of TPC was observed in B. pinnatifolium (35.94 mg GAE g −1 ) while B. microcarpum possessed the highest TFC (39.21 mg RE g −1 ). Seventy four compounds were detected in B. microcarpum, 70 in B. brachyactis, 66 in B. sayai, and 51 in B. pinnatifolium . Quinic acid, chlorogenic acid, pantothenic acid, esculin, isoquercitrin, rutin, apigenin, and scopoletin were present in all the four species. This study showed that the four Bunium species are good sources of biologically active compounds with pharmaceutical and nutraceutical potential. Graphical abstract: Unlabelled Image Highlights: B. microcarpum and B. pinnatifolium exhibited strong antioxidant effects. B. brachyactis was a strong enzyme inhibitor. All species showed strong antimicrobial properties against P. mirabilis. B. sayai was the most non-cytotoxic plant. Bunium species are good sources of bio-active compounds.