Mamey sapote fruit and carotenoid formulations derived thereof are dietary sources of vitamin A – A comparative randomized cross-over study. (August 2019)

• Record Type: Journal Article
• Authors: Chacón-Ordóñez, Tania; Esquivel, Patricia; Quesada, Silvia; Jiménez, Randall R.; Cordero, Aracelly; Carle, Reinhold; Schweiggert, Ralf
• Is Part Of: Food research international. Volume 122(2019)
• Page Start: 340
• Page End: 347
• Publication Date: 2019-08
• Subjects: Bioavailability -- Keto-carotenoids -- Mamey sapote -- Retinyl esters -- Sapotexanthin -- Vitamin A; AUC Area under the curve -- BMI Body mass index -- BHT Butylated hydroxytoluene -- LMM Linear mixed effects model -- MeOH Methanol -- MWCF Molecular weight correction factors -- Ph. Eur. Pharmaceutical grade -- TAG Triacylglyceride -- TBME tert-butyl methyl ether -- TRL Triacylglycerol-rich lipoprotein -- RAE Retinol activity equivalents; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2019.04.009
• Languages: English
• ISSNs: 0963-9969

Abstract

Abstract: Mamey sapote is a fruit rich in specific keto-carotenoids, namely sapotexanthin and cryptocapsin. Their chemical structure suggests their provitamin A activity, although their absorption and conversion to vitamin A remained to be demonstrated in humans. Besides structure-related factors, the fruit matrix might also hamper absorption and conversion efficiency. Therefore, we monitored carotenoid and vitamin A levels in triacylglycerol-rich lipoprotein (TRL) fractions in plasma of human participants after consumption of fresh sapote and a carotenoid-rich "matrix-free" formulation derived thereof. A randomized 2-way cross-over study was conducted to compare the post-prandial bioavailability of 0.8 mg sapotexanthin and 1.2–1.5 mg cryptocapsin from the above-mentioned test meals. Seven blood samples were drawn over 9.5 h after test meal consumption. Carotenoids and retinoids were quantitated in TRL fractions using HPLC-DAD. Sapotexanthin was absorbed by all participants from all meals, being ca. 36% more bioavailable from the "matrix-free" formulation (AUCmedian = 73.4 nmol∙h/L) than from the fresh fruit (AUCmedian = 54.0 nmol∙h/L; p ≤ 0.001). Cryptocapsin was only absorbed by 4 of 13 participants. The appearance of retinyl esters was observed in all participants independent of the test meal. Although the fruit matrix hampered carotenoid in vivo-bioavailability from sapote, the fruit clearly represents a valuable source of vitamin A for humans. Graphical abstract: Unlabelled Image Highlights: Post-prandial absorption of sapotexanthin was proven for all participants. Fruit matrix hampers bioavailability of carotenoids from mamey sapote. Mamey sapote fruit constitutes a valuable source of vitamin A for humans.