Intracellular antioxidant activity and apoptosis inhibition capacity of PEF-treated KDHCH in HepG2 cells. (July 2019)

• Record Type: Journal Article
• Authors: Liang, Rong; Cheng, Sheng; Dong, Yifei; Ju, Huapeng
• Is Part Of: Food research international. Volume 121(2019)
• Page Start: 336
• Page End: 347
• Publication Date: 2019-07
• Subjects: Antioxidant peptide -- Pulsed electric field -- Intracellular antioxidant activity -- Apoptosis -- HepG2 cells; PEF Pulsed electric field -- DPPH 2, 2-Diphenyl-1-picrylhydrazyl -- ABTS 2, 2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt -- DMEM Dulbecco's modified Eagle's medium -- FBS Fetal bovine serum -- PBS Phosphate buffered saline -- DCFH-DA 2′, 7′-dichlorofluorescin diacetate -- DMSO Dimethyl sulfoxide -- DMPO 5, 5′-dimethyl-1-pyrroline-N-oxide -- EPR electron electron paramagnetic resonance -- AAPH 2, 2′-azobis(2-amidinopropane) dihydrochloride -- MTT 3-(4, 5-dimethylthiazol-2-y1)-2, 5-diphenyltetrazo lium bromide -- CAA Cellular antioxidant activity -- H2O2 Hydrogen peroxide -- ROS Reactive oxygen species -- MDA Malonaldehyde -- LDH Lactate dehydrogenase -- CAT Catalase -- SOD Superoxide dismutase -- GSH-PX Glutathione peroxidase -- GR Glutathione reductase -- MMP Mitochondrial membrane potential; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2019.03.049
• Languages: English
• ISSNs: 0963-9969

Abstract

Abstract: The effect of pulsed electric field (PEF) treatment on the intracellular antioxidant and apoptotic activity of the peptide Lys-Asp-His-Cys-His (KDHCH) was examined using model HepG2 cells. First, PEF treatment conditions specific for the antioxidant peptide were optimized, and it was found that PEF treatment could enhance DPPH, ABTS and hydroxyl radical scavenging capacity of KDHCH. Second, KDHCH subjected to PEF treatment at 1800 Hz and 15 kV/cm was investigated using various intracellular antioxidant assays. PEF treatment decreased the EC50 value and increased the protective ability of oxidative stress inhibition and reactive oxygen species (ROS) scavenging activity of KDHCH. Furthermore, catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and glutathione reductase (GR) activities of KDHCH-pre-treated HepG2 cells increased significantly compared with those of the H2 O2 damaged group, whereas lactate dehydrogenase (LDH) and malonaldehyde (MDA) content were decreased. PEF-treated KDHCH exhibited an increased capacity to maintain the stability of mitochondrial membrane potential (MMP) and reduced the level of caspase-3. These results indicate that PEF treatment can enhance the intracellular antioxidant activity of KDHCH, which can inhibit the effect of H2 O2 oxidation on HepG2 cells by inhibiting the accumulation of intracellular ROS, regulating antioxidant related enzymes, and blocking the apoptotic mitochondrial pathways activated by ROS. Graphical abstract: Unlabelled Image Highlights: KDHCH could protect HepG2 cells induced by H2 O2 from oxidation. KDHCH could scavenge ROS in HepG2 cells. KDHCH showed antioxidant enzyme regulating capacity in HepG2 cells. KDHCH showed apoptosis inhibition capacity in HepG2 cells induced by H2 O2 . The PEF treatment enhanced the intracellular antioxidant activity of KDHCH.