Human urine metabolomic signature after ingestion of polyphenol-rich juice of purple grumixama (Eugenia brasiliensis Lam.). (June 2019)

• Record Type: Journal Article
• Authors: Teixeira, Luciane de L.; Dörr, Fabiane; Dias, Carlos T.S.; Pinto, Ernani; Lajolo, Franco M.; Villas-Bôas, Silas G.; Hassimotto, Neuza M.A.
• Is Part Of: Food research international. Volume 120(2019)
• Page Start: 544
• Page End: 552
• Publication Date: 2019-06
• Subjects: Anthocyanins -- Ellagitannins -- Metabolomic approach -- Mitochondrial metabolism; GJ purple grumixama juice -- NADH reduced nicotinamide adenine dinucleotide -- ATP adenosine triphosphate -- LC-MS liquid chromatography coupled to mass spectrometry -- HPLC-DAD high-performance liquid chromatography with diode array detector -- CG-MS gas chromatography coupled to mass spectrometry; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2018.11.004
• Languages: English
• ISSNs: 0963-9969

Abstract

Abstract: Polyphenol intake has been associated with health promotion because of its interaction with several metabolic pathways. This study investigates changes in the urine metabolome following acute intake of polyphenol-rich juice, purple grumixama juice. Grumixama ( Eugenia brasiliensis Lam.) is a cherry native to Brazil that is known to be a rich source of anthocyanins and ellagitannins. In this research 15 healthy subjects consumed a single dose of grumixama juice. Urine samples were collected before grumixama juice intake, 0–1, 1–2, 2–4 h, with fasting at 24 h after intake. Plasma samples were also collected before intake, 30′ and at 1 h, 2 h and 4 h, with fasting at 24 h after juice intake. The urine primary metabolites were analysed by a metabolomic approach using gas chromatography mass spectrometry with methyl chloroformate derivatisation for amino acids and organic acids. Also, an oxygen radical absorbance capacity method was carried out to evaluate the plasma samples antioxidant capacity changes. Subjects showed increase in plasma antioxidant capacity after juice intake ( p -values < .05). A total of 114 metabolites were assessed in urine (1–2 h and 2–4 h), including 17 amino acids, 47 organic acids and several other metabolites. Among the 114 metabolites, 25 were significantly changed during the first 4 h following juice intake, as shown by the Orthogonal Partial Least Squares Discriminant Analysis (0.5 > p(corr) > 0.3) and univariate analysis ( p -values < .05). Some metabolites were related to mitochondrial metabolism, such as glyoxylic acid and oxalic acid. Metabolites related to amino acid metabolism were also changed, such as beta-alanine, l -phenylalanine andl -tyrosine. In conclusion, results suggest that acute intake of grumixama juice could affect amino acid metabolism and mitochondrial metabolism, but the related health implications should be explored in further studies using additional approaches. Graphical abstract: Unlabelled Image Highlights: Urine was analysed to assess the effect of polyphenol-rich juice intake on urinary metabolome in healthy volunteers. Grumixama juice intake influences amino acid metabolism and mitochondrial metabolism. Grumixama juice intake increases plasma antioxidant capacity over 24 h.