Use of TD-GC–TOF-MS to assess volatile composition during post-harvest storage in seven accessions of rocket salad (Eruca sativa). (1st March 2016)

• Record Type: Journal Article
• Authors: Bell, Luke; Spadafora, Natasha D.; Müller, Carsten T.; Wagstaff, Carol; Rogers, Hilary J.
• Is Part Of: Food chemistry. Volume 194(2016)
• Page Start: 626
• Page End: 636
• Publication Date: 2016-03-01
• Subjects: 1-Isothiocyanato-3-methylbutane (PubChem CID: 79086) -- Hexyl-isothiocyanate (PubChem CID: 78120) -- Pentyl isothiocyanate (PubChem CID: 69415) -- Iberverin (PubChem CID: 62351) -- (E)-4-oxohex-2-enal (PubChem CID: 6365145) -- 1-Penten-3-ol (PubChem CID: 12020) -- 1-Penten-3-one (PubChem CID: 15394) -- 2-Methyl-2-butenal (PubChem CID: 5321950) -- (E)-2-pentenal (PubChem CID: 5364752) -- (E, E)-2, 4-hexadienal (PubChem CID: 637564); Eruca sativa -- Diplotaxis tenuifolia -- Brassicaceae -- Rocket salad -- Rucola -- Gas chromatography mass spectrometry -- Volatile organic chemicals -- Isothiocyanates; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2015.08.043
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: We present a robust method for VOC analysis from rocket salad packaging headspace. TD-GC–TOF-MS putatively identified 39 volatile compounds. VOC profiles for Eruca sativa varied significantly between accessions. Isothiocyanate compounds degrade significantly over shelf-life. Changes in VOC profiles could provide a useful tool for assessment of leaf quality. Abstract: An important step in breeding for nutritionally enhanced varieties is determining the effects of the post-harvest supply chain on phytochemicals and the changes in VOCs produced over time. TD-GC–TOF-MS was used and a technique for the extraction of VOCs from the headspace using portable tubes is described. Forty-two compounds were detected; 39 were identified by comparison to NIST libraries. Thirty-five compounds had not been previously reported in Eruca sativa. Seven accessions were assessed for changes in headspace VOCs over 7 days. Relative amounts of VOCs across 3 time points were significantly different – isothiocyanate-containing molecules being abundant on 'Day 0'. Each accession showed differences in proportions/types of volatiles produced on each day. PCA revealed a separation of VOC profiles according to the day of sampling. Changes in VOC profiles over time could provide a tool for assessment of shelf life.