Comparison of two Analytical Methods (ELISA and LC-MS/MS) for Determination of Aflatoxin B1 in Corn and Aflatoxin M1 in Milk. (2015)

• Record Type: Journal Article
• Authors: Stefanovic, Srdjan; Spiric, Danka; Petronijevic, Radivoj; Trailovic, Jelena Nedeljkovic; Milicevic, Dragan; Nikolic, Dragica; Jankovic, Sasa
• Is Part Of: Procedia food science. Volume 5(2015)
• Page Start: 270
• Page End: 273
• Publication Date: 2015
• Subjects: aflatoxin B1 -- aflatoxin M1 -- corn -- milk -- ELISA -- LC-MS/MS; Food -- Periodicals; Food -- Congresses; Food industry and trade -- Periodicals; Food industry and trade -- Congresses; Food; Food industry and trade; Conference proceedings; Periodicals; 664.005
• DOI: 10.1016/j.profoo.2015.09.077
• Languages: English
• ISSNs: 2211-601X

Abstract

Abstract: The aim of this paper is to assess the closeness of agreement between results of ELISA and LC-MS/MS methods for determination of aflatoxin B1 in corn and aflatoxin M1 in milk. Samples of corn (n=100) and milk (n=250) were simultaneously analyzed using ELISA and LC-MS/MS methods, after the severe drought that affected Serbia in summer 2012 resulting in occurrence of aflatoxin B1 in corn and aflatoxin M1 in milk. Regression analysis showed higher level of agreement between aflatoxin B1 samples (R 2 =0.994), compared to aflatoxin M1 samples (R 2 =0.920). However, both techniques were satisfactory in meeting the requirements for official control purposes.