Different genetic responses to oenological conditions between a flocculent wine yeast and its FLO5 deleted strain: Insights from the transcriptome. (December 2018)

• Record Type: Journal Article
• Authors: Di Gianvito, Paola; Tesnière, Catherine; Suzzi, Giovanna; Blondin, Bruno; Tofalo, Rosanna
• Is Part Of: Food research international. Volume 114(2018)
• Page Start: 178
• Page End: 186
• Publication Date: 2018-12
• Subjects: Fermentation kinetics -- Genome wide expression profiles -- Saccharomyces cerevisiae -- Flocculation -- FLO5 deletion -- Oenological conditions; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2018.07.061
• Languages: English
• ISSNs: 0963-9969

Abstract

Abstract: During winemaking Saccharomyces cerevisiae strains are exposed continuously to environmental changes and this microorganism responds modifying its transcriptional profile. Yeast flocculation is considered a social trait that allows the cells to escape from hostile conditions by sedimentation. This behaviour is based on the self-interaction of flocculins, proteins encoded by FLO family genes. These are considered responsible of the facultative-helping type cooperation and were designed as green-beard genes. In order to understand the role of flocculation to stress response, the genome wide expression analysis of a wine flocculent S. cerevisiae F6789A strain and its FLO 5 deleted strain (F6789A-Δ flo 5) were determined, using DNA microarray technology. Results highlighted that F6789A strain showed a shorter lag phase in winemaking condition. The comparison of transcriptomic profiles underlined that, while F6789A-Δ flo 5 strain seemed engaged in the re-organization of the cell wall and in finding different adhesion ways, F6789A strain presented an up-regulation of genes involved in stress response and higher alcohol production. Graphical abstract: Highlights: FLO 5 gene influences fermentation kinetics. In F6789-Δ Flo 5 genes involved in ergosterol biosynthesis were upregulated. Differential expression of genes involved in cell adhesion and stress response. Overexpression of genes involved in the Ehrlich pathway in the wild type.