Gastrointestinal digestates of Grana Padano and Trentingrana cheeses promote intestinal calcium uptake and extracellular bone matrix formation in vitro. (November 2016)

• Record Type: Journal Article
• Authors: De Luca, Paola; Bruschi, Sara; Maggioni, Margherita; Stuknytė, Milda; Cattaneo, Stefano; Bottani, Michela; Fiorilli, Amelia; Rossi, Filippo; De Noni, Ivano; Ferraretto, Anita
• Is Part Of: Food research international. Volume 89(2016) Part 1
• Page Start: 820
• Page End: 827
• Publication Date: 2016-11
• Subjects: ACE Angiotensin-converting enzyme -- CPPs Casein phosphopeptides -- CN Casein -- CTR Control -- CTR plus Positive control -- Dex Dexamethasone -- FA Formic acid -- FBS Fetal Bovine Serum -- FURA-2/AM Fura-2 acetoxymethyl ester -- GP Grana Padano -- HR-MS High Resolution Mass Spectrometry -- IMDM Iscove's Modified Dulbecco's Medium -- ICP-OES Inductively coupled plasma optical emission spectroscopy -- KRH Krebs Ringer HEPES buffer -- MTT 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide -- PR Parmigiano Reggiano -- RPMI Roswell Park Memorial Institute medium -- TEER Transepithelial Electrical Resistance -- TN Trentingrana -- TRPV6 Transient Receptor Potential cation channel subfamily V member 6 -- UPLC Ultra Performance Liquid Chromatography -- β-CN β-Casein; Bone cells -- Calcium -- Casein phosphopeptides -- Cheese digestate -- Co-culture cells; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2016.10.008
• Languages: English
• ISSNs: 0963-9969

Abstract

Abstract: In the present work, Grana Padano (GP) and Trentingrana (TN) cheeses at different ripening time were in vitro digested. To study calcium uptake and utilization, the intact digestates (selected doses that do not alter cell viability and Transepithelial Electrical Resistance) were administered to Caco2/HT-29 70/30 cells, cultured on a semipermeable membrane in transwells, as a model of human intestinal epithelium. Intact digestates as well as the whole basolateral solutions (mimicking the passage of digestates through intestinal cells before reaching the blood flow and bone) in parallel were further administered to human osteoblast-like cells SaOS-2 to study the extracellular bone matrix formation. In vitro digestates deriving from GP and TN promoted calcium uptake and extracellular bone matrix formation independently of both the cheese type and its ripening period (13, 19 or 26 months). The present study reports the ability of whole digestates of GP and TN cheeses to improve intestinal calcium absorption and bone matrix formation in vitro . Once fully explored at bone level, this finding could better support the role of cheese in ameliorating calcium deficiencies and associated diseases in vivo . Graphical abstract: Highlights: In vitro cheese digestates were used for calcium uptake and utilization studies. Digestates promoted calcium uptake and extracellular bone matrix formation in vitro . The reported digestate effects did not depend on cheese type and ripening time.