Dihydrocaffeic acid, a major microbial metabolite of chlorogenic acids, shows similar protective effect than a yerba mate phenolic extract against oxidative stress in HepG2 cells. (September 2016)

• Record Type: Journal Article
• Authors: Baeza, Gema; Sarriá, Beatriz; Mateos, Raquel; Bravo, Laura
• Is Part Of: Food research international. Volume 87(2016:Sep.)
• Page Start: 25
• Page End: 33
• Publication Date: 2016-09
• Subjects: 5-CQA 5-caffeoylquinic acid -- 3, 5-DCQA 3, 5-dicaffeoylquinic acid -- CGAs chlorogenic acids -- CT control -- DCFH-DA dichlorofluorescein-2′7′-diacetate -- DMSO dimethyl sulphoxide -- DNPH 2, 4-dinitrophenlyhydrazine -- DHCA dihydrocaffeic acid -- DHFA dihydroferulic acid -- FBS foetal bovine serum -- GPx glutathione peroxidase -- GR glutathione reductase -- GSH reduced glutathione -- LDH lactate dehydrogenase -- MDA malondialdehyde -- NADH nicotine adenine dinucleotide reduced -- NADPH nicotine adenine dinucleotide phosphate reduced -- OPT o-phthalaldehyde -- PBS phosphate-buffered saline -- ROS reactive oxygen species -- SD standard deviation -- SDS sodium dodecyl sulphate -- t-BOOH tert-butylhydroperoxide -- YMPE yerba mate phenolic extract; Yerba mate -- Polyphenols -- Microbial metabolites -- Dihydrocaffeic acid -- Oxidative stress; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2016.06.011
• Languages: English
• ISSNs: 0963-9969

Abstract

Abstract: The hepatoprotective effect of a yerba mate phenolic extract (YMPE), rich in chlorogenic acids, and its main circulating metabolites dihydrocaffeic (DHCA) and dihydroferulic (DHFA) acids were assessed in human hepatoma HepG2 cells subjected to oxidative damage induced by tert -butylhydroperoxide ( t -BOOH). Direct treatment of HepG2 cells with realistic concentrations of YMPE (1, 10 and 50 μg/mL), DHCA or DHFA (0.2, 1, 10 μM) for 20 h was not cytotoxic and significantly decreased ROS generation. Pre-treatment with YMPE and DHCA prevented the cytotoxicity and macromolecular damage induced by t -BOOH. Moreover, decreased levels of reduced glutathione (GSH), and increased ROS levels and antioxidant enzyme activity induced by t -BOOH were dose-dependently recovered. DHFA only showed a slight protection against cell cytotoxicity, lipid oxidation and GSH depletion. In conclusion, YMPE and one of its major microbial metabolites, DHCA, confer significant protection against oxidative damage, adding evidences to the beneficial health effects associated with mate intake. Graphical abstract: Highlights: YMPE and their major microbial metabolites are not cytotoxic to HepG2 cells. Direct treatment with YMPE, DHCA and DHFA decreases ROS generation in vitro . YMPE and its metabolite DHCA show similar protective effect against oxidative stress. DHCA could strengthen the effect of yerba mate at physiological concentrations. The methylated metabolite DHFA was less effective than DHCA.