Orange juice affects acylcarnitine metabolism in healthy volunteers as revealed by a mass-spectrometry based metabolomics approach. (May 2018)

• Record Type: Journal Article
• Authors: Moreira, Vanessa; Brasili, Elisa; Fiamoncini, Jarlei; Marini, Federico; Miccheli, Alfredo; Daniel, Hannelore; Lee, Jennifer Ji Hye; Hassimotto, Neuza Mariko Aymoto; Lajolo, Franco Maria
• Is Part Of: Food research international. Volume 107(2018)
• Page Start: 346
• Page End: 352
• Publication Date: 2018-05
• Subjects: Orange juice -- Dried blood spots -- Targeted metabolic profile -- Acylcarnitines -- Phosphatidycholines -- LC-MS -- GC–MS; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2018.02.046
• Languages: English
• ISSNs: 0963-9969

Abstract

Abstract: Citrus juices, especially orange juice, constitute rich sources of bioactive compounds with a wide range of health-promoting activities. Data from epidemiological and in vitro studies suggest that orange juice (OJ) may have a positive impact on lipid metabolism. However, the effect of orange juice intake on blood lipid profile is still poorly understood. We have used two different blood samples, Dried Blood Spots (DBS) and plasma, to assess the effect of two-week orange juice consumption in healthy volunteers by a mass-spectrometry based metabolomics approach. DBS were analysed by liquid chromatography mass spectrometry (LC-MS) and plasma samples were analysed by the gas chromatography mass spectrometry (GC–MS). One hundred sixty-nine lipids including acylcarnitines (AC), lysophosphatidylcholines (LysoPC), (diacyl- and acyl-alkyl-) phosphatidylcholines (PC aa and PC ae) and sphingomyelins (SM) were identified and quantified in DBS. Eighteen fatty acids were identified and quantified in plasma. Multivariate analysis allowed to identify an increase in C3:1, C5-DC(C6-OH), C5-M-DC, C5:1-DC, C8, C12-DC, lysoPC18:3, myristic acid, pentadecanoic acid, palmitoleic and palmitic acid and a decrease in nervonic acid, C0, C2, C10, C10:1, C16:1, C16-OH, C16:1-OH, C18-OH, PC aa C40:4, PC ae C38:4, PC ae C42:3, PC ae C42:4 and cholesterol levels after orange juice intake. A two-week period of orange juice intake could affect fatty acids β-oxidation through mitochondrial and peroxisomal pathways, leading to an increase of short-chain acylcarnitines and a decrease of medium and long-chain acylcarnitines. This is the first report analyzing the effect of orange juice intake in healthy volunteers using a dried blood spot-based metabolomics approach. Graphical abstract: Highlights: DBS and plasma were analysed to assess the effect of orange juice intake on lipid profile in healthy volunteers. A metabolomics approach based on LC-MS and GC-MS was used. Orange juice intake increases short-chain acylcarnitines and decreases medium and long-chain acylcarnitines. Orange juice intake could affect fatty acids β-oxidation through mitochondrial and peroxisomal pathways.