Wild Lonicera caerulea berry polyphenol extract reduces cholesterol accumulation and enhances antioxidant capacity in vitro and in vivo. (May 2018)

• Record Type: Journal Article
• Authors: Liu, Suwen; You, Lu; Zhao, Yuhua; Chang, Xuedong
• Is Part Of: Food research international. Volume 107(2018)
• Page Start: 73
• Page End: 83
• Publication Date: 2018-05
• Subjects: Cyanidin-3-glucoside (PubChem CID: 441667) -- Cyanidin-3, 5-diglucoside (PubChem CID: 441688) -- Peonidin-3-glucoside (PubChem CID: 443654) -- Pelargonidin-3-glucoside (PubChem CID: 443648) -- Cyanidin-3-rutinoside (PubChem CID: 44256716) -- Peonidin-3-rutinoside (PubChem CID: 44256842) -- Delphinidin-3-rutinoside (PubChem CID: 44256887) -- Catechin (PubChem CID: 9064) -- Chlorogenic acid (PubChem CID: 1794427) -- Neochlorogenic acid (PubChem CID: 5280633) -- Vanillic acid (PubChem CID: 8468) -- Quercetin-3-O-rutinoside (PubChem CID: 5280805) -- Hyperoside (PubChem CID: 5281643) -- Quercetin-3-O-glucoside (PubChem CID: 25203368) -- 3, 5-Dicaffeoylquinic acid (PubChem CID: 6474310) -- Luteolin-7-O-glucoside (PubChem CID: 5280637); Wild Lonicera caerulea berry -- Hypocholesterolemic activity -- Reduced absorption -- Polyphenols -- Antioxidant; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2018.02.016
• Languages: English
• ISSNs: 0963-9969

Abstract

Abstract: The hypocholesterolemic effect of Lonicera caerulea berry extract rich in polyphenols (LCBP) on high cholesterol-induced hypercholesterolemia and lipoprotein metabolite changes was examined in Caco-2 cells and rats. Cyanidin-3-glucoside, catechin, and chlorogenic acid are the major phenolic components of LCBP. The cholesterol-reducing effect and antioxidant capacity of these components were compared in Caco-2 cells. LCBP (80 μg/mL) and cyanidin-3-glucoside, catechin, and chlorogenic acid (50 μM) were found to be effective (p < 0.05). Rats were fed a high cholesterol diet (HCD) with or without LCBP supplementation (75, 150, and 300 mg/kg body weight intragastrically once daily) for 12 weeks. Compared with the HCD control group, LCBP supplementation at 150 and 300 mg/kg decreased the levels of TC, TG, and LDL-C, but increased that of HDL-C. LCBP treatment promoted greater neutral and acidic sterol excretion (p < 0.05) and improved the antioxidant capacity of the colon tissue, colon contents, and blood. Moreover, trimethylamine N-oxide (TMAO) levels were decreased in serum (p < 0.05). NPC1L1, ACAT2, and MTP mRNA and protein expression were reduced and ABCG5/8 expression was increased (p < 0.05) after LCBP treatment. Our results suggest that LCBP could be used as a functional food for the prevention and treatment of diseases related to excessive cholesterol accumulation. Graphical abstract: LCBP inhibited cholesterol absorption via down-regulated cholesterol absorption gene mRNA and protein expression and increased antioxidant capacity in vitro and vivo. Highlights: The cholesterol-lowering activity of LCBP was investigated in rats by HCD. LCBP normalized abnormal serum TC, TG, HDL-C, and LDL-C levels. The cholesterol-reducing effect of C-3-G, catechin, and CA were compared in cells. LCBP downregulates cholesterol absorption gene. LCBP improved colon and serum antioxidant capacity.