UPLC/QTOF/MS profiling of two Psidium species and the in-vivo hepatoprotective activity of their nano-formulated liposomes. (March 2018)

• Record Type: Journal Article
• Authors: Saber, Fatema R.; Abdelbary, Ghada A.; Salama, Maha M.; Saleh, Dalia O.; Fathy, Magda M.; Soliman, Fathy M.
• Is Part Of: Food research international. Volume 105(2018)
• Page Start: 1029
• Page End: 1038
• Publication Date: 2018-03
• Subjects: ALP alkaline phosphatase -- ALT alanine amino transferase -- AST aspartate amino transferase -- b. wt body weight -- CH cholesterol -- CME chloroform: methanol (80: 20) extract -- GSH glutathione -- HCV hepatitis C virus -- PG and PC nano-liposomes containing P. guajava and P. cattleianum leaf extracts respectively -- Ph C phosphatidyl choline -- RDA Retro Diels-Alder -- ROS reactive oxygen species -- MDA malondialdehyde -- UPLC-PDA-ESI-qTOF-MSn ultra-performance liquid chromatography coupled to photodiode array detector, mass spectroscopy with electrospray ionization source and quadrupole time of flight analyzer; UPLC/MS -- Metabolic profiling -- Psidium guajava -- Psidium cattleianum -- Liposome -- Hepatoprotective; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2017.12.042
• Languages: English
• ISSNs: 0963-9969

Abstract

Abstract: Liver diseases are major health problem in Egypt influencing lifestyle and economy. The demand for nutraceutical hepatoprotective agents is crucial to ameliorate the side effects of synthetic drugs. The present study aims to evaluate antioxidant and hepatoprotective activities of extracts of Psidium guajava L. and Psidium cattleianum Sabine leaves and their nano-formulated liposomes against paracetamol-induced liver damage in rats. Secondary metabolites profile of P. guajava and P. cattleianum leaves was investigated using UPLC-PDA-ESI-qTOF-MSn. The nano-liposomes containing Psidium extracts were prepared using thin film hydration method. Biochemical analysis was based on monitoring serum levels of AST, ALT, ALP and total bilirubin. The liver homogenate was used for determination of GSH and MDA. Histopathological alterations were also studied. Metabolic profiling revealed qualitative differences between the two investigated species providing a comprehensive map for the metabolites present in P. guajava and P. cattleianum leaves cultivated in Egypt. The identified metabolites belong to different phytochemical classes; polyphenolics, flavonoids, triterpenes and meroterpenoids. Significant hepatoprotective effects were observed as evident from the decreased levels of AST, ALT, ALP, MDA and total bilirubin as well as restoration of decreased GSH level in the two studied Psidium extracts (250, 500 mg/kg b. wt) and their respective nano-liposomes (500 mg/kg b. wt), when compared to the diseased group. Nano-liposomes of Psidium guajava leaves (500 mg/kg b. wt) greatly restored the normal architecture of the liver in the histopathological study, as regards to standard silymarin. The present study verified the effectiveness of Psidium guajava and Psidium cattleianum leaves extracts and their nano-liposomes in ameliorating the paracetamol-induced hepatotoxicity in rats. Graphical abstract: Highlights: Metabolic profile of P. guajava and P. cattleianum leaves was investigated using UPLC-PDA-ESI-qTOF-MSn. Polyphenolics, flavonoids, triterpenes and meroterpenoids were identified in both plant extracts. Nano-formulated liposomes were prepared using thin film hydration technique. Psidium extracts and their nano-liposomes significantly ameliorated paracetamol-induced hepatotoxicity in rats.