HPLC-DAD-APCI/ESI-MSn analysis of carotenoids and α-tocopherol in Costa Rican Acrocomia aculeata fruits of varying maturity stages. (March 2018)

• Record Type: Journal Article
• Authors: Schex, Roland; Lieb, Veronika M.; Jiménez, Víctor M.; Esquivel, Patricia; Schweiggert, Ralf M.; Carle, Reinhold; Steingass, Christof B.
• Is Part Of: Food research international. Volume 105(2018)
• Page Start: 645
• Page End: 653
• Publication Date: 2018-03
• Subjects: APCI atmospheric pressure chemical ionisation -- BHA butylated hydroxyanisole -- BHT butylated hydroxytoluene -- CID collision induced dissociation -- ESI electrospray ionisation -- HCA hierarchical cluster analysis -- HPLC-DAD high performance liquid chromatography-diode array detection -- LOD limit of detection -- LOQ limit of quantitation -- MSn multiple-stage mass spectrometry -- PCA principal component analysis -- tBME tert-butyl methyl ether; Macauba -- Coyol -- Palm fruit -- Underutilised crop -- Carotenoid precursor -- Xanthophyll cycle; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2017.11.041
• Languages: English
• ISSNs: 0963-9969

Abstract

Abstract: Carotenoids and tocopherols were characterised in the meso- and exocarp of wild-growing Costa Rican Acrocomia aculeata fruits. Comprehensive profiling of these lipophilic micronutrients in fruits of three varying maturity stages was conducted for the first time. A method for the simultaneous extraction and quantitation of carotenoids and α-tocopherol was developed and validated. Detailed HPLC-DAD-APCI/ESI-MS n analyses enabled the identification of α-tocopherol and 25 carotenoids. The latter comprised antheraxanthin, β-carotene, lutein, luteoxanthin, neoxanthin, phytoene, phytofluene, violaxanthin, zeaxanthin, and several ( Z )-isomers of the aforementioned compounds. Quantitation by HPLC-DAD/FLD revealed total carotenoid concentrations of 872 ± 178 and 3075 ± 407 μg/100 g fresh weight in the meso- and exocarp of fully ripe fruits, respectively. In both fruit fractions, progressing maturation resulted in the accumulation of phytoene, phytofluene, (all- E )-zeaxanthin, (all- E )-antheraxanthin, and (all- E )-violaxanthin. Carotenoid profiling was supported by multivariate data analysis. Carotenoid precursors and xanthophyll cycle pigments characterised Macauba fruits of full maturity. Graphical abstract: Highlights: Simultaneous extraction of carotenoids and α-tocopherol from Macauba fruits Identification of 25 carotenoids and α-tocopherol by HPLC-DAD-APCI/ESI-MS n Quantitation in the meso- and exocarp of three progressing maturity stages PCA was used for pattern recognition among the Macauba fruit samples.