Universal mini COI barcode for the identification of fish species in processed products. (March 2018)

• Record Type: Journal Article
• Authors: Sultana, Sharmin; Ali, Md. Eaqub; Hossain, M.A. Motalib; Asing, ; Naquiah, Nina; Zaidul, I.S.M.
• Is Part Of: Food research international. Volume 105(2018)
• Page Start: 19
• Page End: 28
• Publication Date: 2018-03
• Subjects: Fraud labelling -- Fish mini barcode -- Processed fish and surimi products -- DNA breakdown -- Forensic studies; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2017.10.065
• Languages: English
• ISSNs: 0963-9969

Abstract

Abstract: Species substitution, the use of a low value fish in place of a high value fish, is the biggest problem in international trade and the leading cause of fraud in the fisheries arena sector. Current DNA barcoding systems have partly solved this problem but also failed in many instances to amplify PCR targets from highly processed products because of the degradation of a longer barcode marker (~ 650 bp). In the present study, a novel mini barcode marker (295 bp) was developed to discriminate fish species in raw and processed states forms. The barcode primers were cross-tested against 33 fish species and 15 other animal species and found to be universal for all the tested fish varieties. When 20 commercial fish products of five different categories were screened, all commercial fish sample yielded positive bands for the novel fish barcode. PCR product was sequenced to retrieve the species IDs that reflected 55% (11/20) of Malaysian fish products were mislabeled. Graphical abstract: Highlights: Novel COI mini barcode (295 bp) to authenticate fish species. It was validated against 33 fish and 15 non-fish species. It is suitable to be used as a universal fish positive control under mixed matrices. The screening of 22 Malaysian fish products revealed 55% fraud labelling.