Antimicrobial peptide AMPNT-6 from Bacillus subtilis inhibits biofilm formation by Shewanella putrefaciens and disrupts its preformed biofilms on both abiotic and shrimp shell surfaces. (December 2017)

• Record Type: Journal Article
• Authors: Deng, Qi; Pu, Yuehua; Sun, Lijun; Wang, Yaling; Liu, Yang; Wang, Rundong; Liao, Jianmeng; Xu, Defeng; Liu, Ying; Ye, Riying; Fang, Zhijia; Gooneratne, Ravi
• Is Part Of: Food research international. Volume 102(2017)
• Page Start: 8
• Page End: 13
• Publication Date: 2017-12
• Subjects: Bacillus subtilis -- Antimicrobial lipopeptide -- Shewanella putrefaciens -- Anti-adhesion -- Biofilm removal; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2017.09.057
• Languages: English
• ISSNs: 0963-9969

Abstract

Abstract: Shewanella putrefaciens biofilm formation is of great concern for the shrimp industry because it adheres easily to food and food-contact surfaces and is a source of persistent and unseen contamination that causes shrimp spoilage and economic losses to the shrimp industry. Different concentrations of an antimicrobial lipopeptide, the fermentation product of Bacillus subtilis, AMPNT-6, were tested for the ability to reduce adhesion and disrupt S . putrefaciens preformed biofilms on two different contact surfaces (shrimp shell, stainless steel sheet). AMPNT-6 displayed a marked dose- and time-dependent anti-adhesive effect > biofilm removal. 3MIC AMPNT-6 was able both to remove biofilm and prevent bacteria from forming biofilm in a 96-well polystyrene microplate used as the model surface. 2MIC AMPNT-6 prevented bacteria from adhering to the microplate surface to form biofilm for 3 h and removed already existing biofilm within 24 h. Secretion of extracellular polymeric substances incubated in LB broth for 24 h by S . putrefaciens was minimal at 3 × MIC AMPNT-6. Scanning electron microscopy showed that damage to S . putrefaciens bacteria by AMPNT-6 possibly contributed to the non-adherence to the surfaces. Disruption of the mature biofilm structure by AMPNT-6 contributed to biofilm removal. It is concluded that AMPNT-6 can be used effectively to prevent attachment and also detach S . putrefaciens biofilms from shrimp shells, stainless steel sheets and polystyrene surfaces. Graphical abstract: Highlights: AMPNT-6 was used to control spoilage bacterial biofilm on shrimp shells. S . putrefaciens biofilm formed more readily on stainless steel than shrimp shells. The biofilm of S . putrefaciens SL-3 can be removed by AMPNT-6. AMPNT-6 displayed a marked dose-dependent anti-adhesive effect > biofilm removal. SEM clearly showed AMPNT-6 induced structural damage to biofilms.