Comparative evaluation of three different ELISA assays and HPLC-ESI-ITMS/MS for the analysis of Nε-carboxymethyl lysine in food samples. (15th March 2018)

• Record Type: Journal Article
• Authors: Gómez-Ojeda, Armando; Jaramillo-Ortíz, Sarahi; Wrobel, Katarzyna; Wrobel, Kazimierz; Barbosa-Sabanero, Gloria; Luevano-Contreras, Claudia; de la Maza, Maria Pia; Uribarri, Jaime; del Castillo, Ma. Dolores; Garay-Sevilla, Ma. Eugenia
• Is Part Of: Food chemistry. Volume 243(2018)
• Page Start: 11
• Page End: 18
• Publication Date: 2018-03-15
• Subjects: Nε-carboxymethyl lysine -- Food -- ELISA -- Liquid chromatography – mass spectrometry; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2017.09.098
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: CML determined in twenty food items typically consumed in Mexico. Performance of three ELISA assays and HPLC-ESI-ITMS/MS comparatively evaluated. Feasibility of ELISA assays for CML determination in food demonstrated. CML in food: beef/bacon > chicken>fish > dairy products > grain products > fruit/vegetable. CML confirmed as a chemical indicator of advanced glycation end products in food. Abstract: N ε -carboxymethyl-lysine (CML) is measured in food, but there is a controversy concerning the most convenient yet reliable method(s) for this task. This work compares three different ELISA assays and HPLC-ESI-ITMS/MS for the analysis of CML in several food items. The four methods showed the same decreasing order of CML concentration: beef, bacon > chicken > fish > dairy products > grain products > fruits/vegetables. HPLC-ESI-ITMS/MS results highly correlated with those obtained by ELISA performed with monoclonal CML-antibody (β = 0.98, p < 0.0001) whereas My Bio Source® kit results were not correlated with those provided by Lamider®. Small differences of CML concentrations in food items prepared by different culinary treatment were clearly distinguished by HPLC-ESI-ITMS/MS, but could not always be detected by ELISA. This work demonstrates a reasonable relationship between CM determined by ELISA and HPLC-ESI-ITMS/MS and therefore supports the implementation of ELISA in food CML/AGEs screening.