Liquid chromatography-high resolution mass spectrometry for the analysis of phytochemicals in vegetal-derived food and beverages. (October 2017)

• Record Type: Journal Article
• Authors: La Barbera, Giorgia; Capriotti, Anna Laura; Cavaliere, Chiara; Montone, Carmela Maria; Piovesana, Susy; Samperi, Roberto; Zenezini Chiozzi, Riccardo; Laganà, Aldo
• Is Part Of: Food research international. Volume 100 Part 1(2017)
• Page Start: 28
• Page End: 52
• Publication Date: 2017-10
• Subjects: Phytochemicals -- Food analysis -- High resolution mass spectrometry -- High resolution liquid chromatography -- Phenolics -- Polyphenols -- Metabolomics; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2017.07.080
• Languages: English
• ISSNs: 0963-9969

Abstract

Abstract: The recent years witnessed a change in the perception of nutrition. Diet does not only provide nutrients to meet the metabolic requirements of the body, but it also constitutes an active way for the consumption of compounds beneficial for human health. Fruit and vegetables are an excellent source of such compounds, thus the growing interest in characterizing phytochemical sources, structures and activities. Given the interest for phytochemicals in food, the development of advanced and suitable analytical techniques for their identification is fundamental for the advancement of food research. In this review, the state of the art of phytochemical research in food plants is described, starting from sample preparation, throughout extract clean-up and compound separation techniques, to the final analysis, considering both qualitative and quantitative investigations. In this regard, from an analytical point of view, fruit and vegetable extracts are complex matrices, which greatly benefit from the use of modern hyphenated techniques, in particular from the combination of high performance liquid chromatography separation and high resolution mass spectrometry, powerful tools which are being increasingly used in the recent years. Therefore, selected applications to real samples are presented and discussed, in particular for the analysis of phenols, polyphenols and phenolic acids. Finally, some hot points are discussed, such as waste characterization for high value-compounds recovery and the untargeted metabolomics approach. Graphical abstract: Highlights: Food is not only source of nutrients but also of beneficial compounds for health. Phytochemicals are nutraceuticals commonly found in food of vegetable origin. Dedicated analytical workflows are needed to identify and quantify phytochemicals. Modern approaches are described both for food and wastes.