Effect of explosion-puffed coffee on locomotor activity and behavioral patterns in Drosophila melanogaster. (October 2017)

• Record Type: Journal Article
• Authors: Ko, Bong Soo; Ahn, So Hyun; Noh, Dong Ouk; Hong, Ki-Bae; Han, Sung Hee; Suh, Hyung Joo
• Is Part Of: Food research international. Volume 100 Part 2(2017)
• Page Start: 252
• Page End: 260
• Publication Date: 2017-10
• Subjects: Explosion puffed coffee -- Decaffeinated coffee -- Locomotor activity -- Drosophila melanogaster; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2017.08.051
• Languages: English
• ISSNs: 0963-9969

Abstract

Abstract: We hypothesized that the administration of explosion-puffed coffee, containing γ-aminobutyric acid (GABA) and 5-hydroxytryptophan (5-HTP), would be associated with a reduction of the caffeine effect on sleep behavior and behavioral patterns, which was investigated in a Drosophila model. The effects of feeding roasted coffee beans (RB), explosion-puffed coffee beans puffed at 0.75 MPa and 0.9 MPa (PB 7.5 and PB 9.0, respectively), or decaffeinated coffee beans (DeRB) on locomotor activity and behavioral patterns of Drosophila was analyzed. In the decreasing order, the total chlorogenic acid (caffeoylquinic acids, CQA) content was PB 7.5 > PB 9.0 > RB. PB content analysis showed high levels of GABA and 5-HTP, compared with that of RB, which corresponded with the sleep-wake behavior of Drosophila . The RB and PB (PB 7.5 and PB 9.0) groups were not significantly different with respect to an activity count during the subjective night and day period compared with the normal controls. Sleep bout numbers of the normal, PB, and DeRB groups showed significant differences as compared with the caffeine and RB groups ( p < 0.05). The PB and DePB groups showed a significantly increased transcript levels for the GABA receptors compared to the caffeine group. The caffeine and RB groups displayed better climbing ability than the other groups, covering an average distance 6 cm in the related test; the average distance covered by the normal, PB 7.5, and DeRB groups was < 4 cm. The normal and DeRB groups showed similar behavior patterns with respect to total distance, velocity, moving, not moving, and meander. However, the PB 7.5 group significantly regulated not moving and meander of flies compared to flies receiving only caffeine and RB. Suppression of the stimulating effect of caffeine by explosion-puffed coffee administration was indicated in the above results, which can be attributed to the increased content of GABA and 5-HTP with explosive puffing process carried out at 0.75 MPa. Results of the underlying mechanism of the behavioral change patterns of explosive puffed with or without caffeine in Drosophila models, transcript level for the Dop1-R1 receptor in caffeine group was significantly higher than normal, PB, and DePB groups. Flies exposed to the caffeine had significantly decreased transcript levels for the GABA receptors. PB 7.5 and DePB showed higher level of GABA content than RB. Graphical abstract: Highlights: Contents of γ-aminobutyric acid (GABA) and 5-hydroxytryptophan (5-HTP) were increased by explosion puffing in coffee. Explosion puffed coffee showed a sleep enhancement effect in Drosophila model. Explosion puffed coffee showed the increase of transcript level on Rdl and GABA receptor.