Fermented pearl millet (Pennisetum glaucum) with in vitro DNA damage protection activity, bioactive compounds and antioxidant potential. (October 2017)

• Record Type: Journal Article
• Authors: Salar, Raj Kumar; Purewal, Sukhvinder Singh; Sandhu, Kawaljit Singh
• Is Part Of: Food research international. Volume 100 Part 2(2017)
• Page Start: 204
• Page End: 210
• Publication Date: 2017-10
• Subjects: Aspergillus sojae -- Pennisetum glaucum -- Antioxidant potential -- Solid state fermentation -- DNA damage protection; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2017.08.045
• Languages: English
• ISSNs: 0963-9969

Abstract

Abstract: In the present study, pearl millet cultivar PUSA-415 was fermented by solid state fermentation (SSF) process using Aspergillus sojae (MTCC-8779) as starter culture. The fermentation was carried out for the period of ten days. The effect of SSF on phenolic content, condensed tannin content, antioxidant potential and DNA damage protection of pearl millet during different fermentation period was determined. Results showed that SSF and thermal processing significantly affect the bioactive profile and antioxidant potential of bio-transformed pearl millet. Extracts prepared from 6th days fermented pearl millet flour exhibited the highest TPC, antioxidant potential and DNA damage protection activity. Qualitative and quantitative analysis of bioactive compounds were done by HPLC. During SSF, production of enzymes (α-amylase, β-glucosidase and xylanase) as well as specific bioactive compounds (ascorbic acid, gallic acid and p-Coumaric acid) was significantly increased. Thus, bio-transformed Aspergillus sojae fermented pearl millet could be used in preparation of functional foods and novel nutraceuticals in health promotions. Chapatti was formulated from unfermented as well as fermented flour and the effect of thermal processing on bioactive compounds and antioxidant potential was studied. Thermal processing resulted in decrease in TPC of both, AFM and UFM by 4.75–16.27% and increase in CTC by 38.52–67.41%. Graphical abstract: Highlights: Pearl millet cultivar fermented with a fungal strain Aspergillus sojae using SSF SSF increased the phenolic content, condensed tannin content, antioxidant potential. 6th day fermented sample had the highest antioxidant potential & DNA protection. HPLC showed ascorbic acid, gallic acid and p-Coumaric acid in fermented samples. SSF resulted in the production of α-amylase, β-glucosidase and xylanase.