Influence of omega-3 PUFAs on the metabolism of proanthocyanidins in rats. (July 2017)

• Record Type: Journal Article
• Authors: Molinar-Toribio, Eunice; Ramos-Romero, Sara; Fuguet, Elisabet; Taltavull, Núria; Méndez, Lucía; Romeu, Marta; Medina, Isabel; Torres, Josep Lluís; Pérez-Jiménez, Jara
• Is Part Of: Food research international. Volume 97(2017)
• Page Start: 133
• Page End: 140
• Publication Date: 2017-07
• Subjects: EC (epi)catechin -- EGC (epi)gallocatechin -- Gluc glucuronyl group -- GSE grape seed extract -- Me methyl group -- MRM multiple reaction monitoring -- PAs proanthocyanidins -- STD standard diet -- Sulf sulfate group -- SPE solid-phase extraction; Polyphenols -- Proanthocyanidins -- Omega-3 polyunsaturated fatty acids -- Bioavailability; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2017.03.046
• Languages: English
• ISSNs: 0963-9969

Abstract

Abstract: Studies of the bioavailability of proanthocyanidins usually consider them independently of other dietary constituents, while there is a tendency in the field of functional foods towards the combination of different bioactive compounds in a single product. This study examined the long-term effects of ω-3 polyunsaturated fatty acids of marine origin on the metabolic fate of grape proanthocyanidins. For this, female adult Wistar-Kyoto rats were fed (18 weeks) with a standard diet supplemented or not with eicosapentaenoic acid/docosahexaenoic acid (1:1, 16.6 g/kg feed), proanthocyanidin-rich grape seed extract (0.8 g/kg feed) or both. A total of 39 microbial-derived metabolites and 16 conjugated metabolites were detected by HPLC-MS/MS either in urine or in the aqueous fraction of feces. An unexpected significant increase in many proanthocyanidin metabolites in urine and feces was observed in the group supplemented with ω-3 polyunsaturated fatty acids group as compared to the animals fed a standard diet, which contains a small amount of polyphenols. However, proanthocyanidin metabolites in rats given ω-3 polyunsaturated fatty acids and grape seed extract did not significantly differ from those in the group supplemented only with grape seed extract. It was concluded that ω-3 polyunsaturated fatty acids collaborate in the metabolism of polyphenols when present at low doses in the feed matrix, while the capacity of ω-3 polyunsaturated fatty acids to induce microbiota transformations when proanthocyanidins are present at high doses is not relevant compared to that of polyphenols themselves. Graphical abstract: Highlights: ω-3 PUFAs influence the metabolism of proanthocyanidins (PAs). ω-3 PUFAs increase metabolites of PAs present in the standard diet. ω-3 PUFAs do not increase PA metabolites when PA are given as a supplement. ω-3 PUFAs do not significantly affect PA metabolism given at high doses.