Development of primer set for the identification of fish species in surimi products using denaturing gradient gel electrophoresis. (September 2017)

• Record Type: Journal Article
• Authors: Noh, Eun Soo; Park, Yeon Jung; Kim, Eun Mi; An, Cheul Min; Park, Jung Youn; Kim, Kyoung-Ho; Song, Jung-Hun; Kang, Jung-Ha
• Is Part Of: Food control. Volume 79(2017:Sep.)
• Page Start: 74
• Page End: 79
• Publication Date: 2017-09
• Subjects: Surimi -- Denaturing gradient gel electrophoresis -- Identification -- Universal primer; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2017.03.024
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: The purpose of this study was to develop a DNA marker for the identification of fish species in processed surimi products. A DNA marker was designed based on the mitochondrial cytochrome c oxidase subunit I gene, and fish species in surimi products were identified using a molecular fingerprinting technique, denaturing gradient gel electrophoresis (DGGE); the results were subjected to sequence-based analysis. The DGGE profiles indicated the presence in surimi products of a greater diversity of fish species than reported previously: 20 species belonging to 16 genera were identified. Therefore, our method facilitates the simple and rapid detection and identification of fish species in seafood products produced from minced fish. Highlights: Efficient marker was developed to identify the fish species from processed food. A method for the identification of fish species using DGGE has been developed. DGGE profiles revealed the presence of a variety of fish species in surimi products.