Characterization of non-dialyzable constituents from cranberry juice that inhibit adhesion, co-aggregation and biofilm formation by oral bacteria. Issue 5 (4th May 2017)

• Record Type: Journal Article
• Authors: Neto, C. C.; Penndorf, K. A.; Feldman, M.; Meron-Sudai, S.; Zakay-Rones, Z.; Steinberg, D.; Fridman, M.; Kashman, Y.; Ginsburg, I.; Ofek, I.; Weiss, E. I.
• Is Part Of: Food & function. Volume 8:Issue 5(2017)
• Page Start: 1955
• Page End: 1965
• Publication Date: 2017-05-04
• Subjects: Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Nutrition -- Periodicals; 664.07
• DOI: 10.1039/c7fo00109f
• Languages: English
• ISSNs: 2042-6496

Abstract

Abstract : A mixture of compounds identified in non-dialyzable fraction from cranberry juice inhibits adhesion, coaggregation and biofilm formation by oral bacteria. Abstract : An extract prepared from cranberry juice by dialysis known as nondialyzable material (NDM) has been shown previously to possess anti-adhesion activity toward microbial species including oral bacteria, uropathogenic Escherichia coli and Helicobacter pylori . Bioassay-guided fractionation of cranberry NDM was therefore undertaken to identify the anti-adhesive constituents. An aqueous acetone-soluble fraction (NDMac) obtained from Sephadex LH-20 inhibited adhesion-linked activities by oral bacteria, including co-aggregation of oral bacteria Fusobacterium nucleatum with Streptococcus sanguinis or Porphyromonas gingivalis, and biofilm formation by Streptococcus mutans . Analysis of NDMac and subsequent subfractions by MALDI-TOF MS and 1 H NMR revealed the presence of A-type proanthocyanidin oligomers (PACs) of 3–6 degrees of polymerization composed of ( epi )catechin units, with some ( epi )gallocatechin and anthocyanin units also present, as well as quercetin derivatives. Subfractions containing putative xyloglucans in addition to the mixed polyphenols also inhibit biofilm formation by S. mutans (MIC = 125–250 μg mL −1 ). These studies suggest that the anti-adhesion activities of cranberry NDM on oral bacteria may arise from a combination of mixed polyphenol and non-polyphenol constituents.